检验医学与临床2024，Vol.21Issue(7)：980-982,986,4.DOI:10.3969/j.issn.1672-9455.2024.07.025
宏基因组测序技术辅助诊断人型支原体及解脲支原体共同感染的成人脑膜炎的临床研究
Clinical study of Mycoplasma hominis and Ureaplasma urealyticum co-infected adult meningitis by metagenomic next-generation sequencing technology assisted diagnosis
摘要
Abstract
Objective To explore the application of metagenomic next-generation sequencing technology(mNGS)in the diagnosis of Mycoplasma humanum and Mycoplasma urealyticum complicating central nervous system infection through the diagnosis and treatment situation in a case of adult meningitis co-infected by My-coplasma hominis and Mycoplasma urealyticum and a review of related literature,so as to improve the clini-cians'diagnosis and treatment level of such rare infectious disease.Methods The clinical diagnosis and treat-ment process in an adult menigngistis patients of Mycoplasma hominis and Mycoplasma urealyticum co-infec-tion admitted and treated in this hospital in June 2021 was summarized."Mycoplasma hominis""Ureaplasma urealyticum""meningitis"and"CSF"served as the English key words to retrieve the PubMed database from January 2000 to June 2021 for conducting the literature review.Results The patient visited the hospital due to severe head trauma and developed intermittent fever postoperatively,the cerebrospinal fluid biochemical tests suggested acute meningitis.After clinical empirical anti-infective treatment with antibacterial drugs such as meropenem,linezolid,which was not effective,the patient still had recurrent fever and the primary foci of in-fection were unclear.The cerebrospinal fluid was detected by mNGS,which reported high sequence numbers of Mycoplasma hominis and Mycoplasma urealyticum,and the patient was then given doxycycline treatment,then the systemic condition was improved and the symptoms reduced after 3 d treatment,and after 7 d treat-ment,the subarachnoid hemorrhage was significantly absorbed,and the bilateral frontotemporal subdural effu-sion gradually decreased to disappear compared with the previous one,and the patient recovered smoothly with no signs of recurrence.A total of 23 case reports were included in the literature retrieval,in which 20 cases of meningitis were caused by Mycoplasma hominis infection and 3 cases were caused by Ureaplasma urealyticum infection.Conclusion With the development of the mNGs technology and its clinical application,the diagnosis and treatment efficiency of infectious diseases with rare pathogens that are susceptible to clinical misdiagnosis has been improved.关键词
人型支原体/解脲支原体/脑膜炎/共同感染/宏基因组二代测序技术Key words
Mycoplasma hominis/Ureaplasma urealyticum/meningitis/co-infection/metagenomic next-generation sequencing分类
医药卫生引用本文复制引用
袁磊,赖兰敏,陈嫱..宏基因组测序技术辅助诊断人型支原体及解脲支原体共同感染的成人脑膜炎的临床研究[J].检验医学与临床,2024,21(7):980-982,986,4.基金项目
江西省教育厅基金项目(GJJ200220) （GJJ200220）
江西省卫生建康委员会科技项目(SKJP220212485). （SKJP220212485）
