检验医学与临床2024，Vol.21Issue(7)：1003-1007,1024,6.DOI:10.3969/j.issn.1672-9455.2024.07.031
环介导等温扩增技术在病原微生物临床检测中的应用
Application of loop-mediated isothermal amplification technique in pathogenic microorganisms clinical detection
摘要
Abstract
Pathogenic microorganisms are one of the important factors threatening human health.Rapid and accurate detection methods are of great significance for the diagnosis of infectious diseases.Loop-mediated isothermal amplification(LAMP)is one of the isothermal amplification methods,which has the advantages of short reaction time,simple operation,high sensitivity,high specificity and low cost,so it is widely used in the rapid diagnosis of infectious diseases.Based on this situation,this paper mainly elaborates the principle and characteristics of LAMP and its application and research progress in the detection of common pathogenic mi-croorganisms in clinic.LAMP technology has been applied to the detection of pathogenic microorganisms with high sensitivity and specificity,but this technology is still prone to produce false positive,complex primer de-sign and other shortcomings.This paper reviews the application and progress of LAMP technology in patho-genic microbial infection in recent years,and outlooks its future development prospect in order to provide a reasonable research direction for the rapid diagnosis of pathogenic microbial infection in the environment with limited resources.关键词
环介导等温扩增技术/核酸检测/病原微生物/核酸扩增/结核分枝杆菌Key words
loop-mediated isothermal amplification technique/nucleic acid test/pathogenic microor-ganism/nucleic acid amplification/mycobacterium tuberculosis分类
医药卫生引用本文复制引用
廖川,梁丽娜,黄少宇,马梦霞,李雪斌,韦贵将..环介导等温扩增技术在病原微生物临床检测中的应用[J].检验医学与临床,2024,21(7):1003-1007,1024,6.基金项目
国家自然科学基金项目(82260418,82060570) （82260418,82060570）
右江民族医学院附属医院高层次人才项目(R202011701) （R202011701）
广西壮族自治区百色市科学研究与技术开发计划项目(百科20232031). （百科20232031）
