河南农业科学2024，Vol.53Issue(3)：103-109,7.DOI:10.15933/j.cnki.1004-3268.2024.03.010
暹罗芽孢杆菌Y-54对番茄叶霉病的生防作用
Biocontrol Effect of Bacillus siamensis Y-54 against Tomato Leaf Mold
摘要
Abstract
In order to clarify the biocontrol effect of Bacillus siamensis Y-54 against tomato leaf mold and provide a scientific basis for the biological control of tomato leaf mold,the inhibitory effect of strain Y-54 on Cladosporium fulvum was measured by confrontation and microscopic observation,and the colonization of antagonistic strain Y-54 in tomato was studied by irrigation method.The growth promoting effect of the strain Y-54 on seed germination and seedling growth of tomato was studied by soaking seed method and filling root method.The control effect was measured by pot experiment.The result showed that strain Y-54 and its fermentation broth and sterile filtrate all had strong inhibitory effects on C.fulvum,with inhibition rates reaching 78.42%,86.42%and 91.26%,respectively.The sterile filtrate could make mycelium of C.fulvum deform,bend,shrink,expand and break.The strain Y-54 could colonize in the roots,stems and leaves of tomato within 30 days.The colonization of the strain Y-54 reached the peak at 15-18 d.The colonization in the root was always higher than that in other parts.Strain Y-54 could effectively promote seed germination.The germination rate of fermentation broth(0×)treatment was the highest,reaching 93.33%.Moreover,it could effectively promote the increase of root length,plant height,fresh weight,chlorophyll and other indexes of seedlings.The growth promoting effect of 0×,10×and 20×fermentation broth was significantly higher than that of the control with sterile water.The control effects of strain Y-54 fermentation broth on tomato leaf mold within 7 days and 14 days were 68.04%and 71.07%,respectively,close to that of phenoxymethyclozole treatment,and significantly higher than that of carbendazim treatment.In summary,the strain Y-54 had good control effect on tomato leaf mold,and it was a biocontrol bacterium worth further development and utilization.关键词
番茄叶霉病/暹罗芽孢杆菌/定殖/促生作用/防治效果Key words
Tomato leaf mold/Bacillus siamensis/Colonization/Growth-promoting effect/Control effect分类
农业科技引用本文复制引用
曾婉宁,王彦譞,王繁珍,陈招荣,刘慧芹,姜永成,艾则麦提·图尔洪,李宝通..暹罗芽孢杆菌Y-54对番茄叶霉病的生防作用[J].河南农业科学,2024,53(3):103-109,7.基金项目
河北省重点研发计划项目(22347402D) （22347402D）
新疆维吾尔自治区二次引进项目(2022008) （2022008）
天津市农业科技成果转化与推广项目(202101080) （202101080）
山东省重点研发计划项目(2023TZXD034) （2023TZXD034）
2023年度新疆维吾尔自治区科技成果转化示范专项 （）
和田地区本级技术研究与开发经费项目(20220219) （20220219）
