中国临床保健杂志2024，Vol.27Issue(1)：106-111,6.DOI:10.3969/J.issn.1672-6790.2024.01.022
多肽CRY-14保护心肌对抗缺血缺氧损伤的作用机制
Study on the mechanism of peptide CRY-14 in protecting myocardium against hypoxic-ischemic injury
摘要
Abstract
Objective To explore the role and mechanism of peptide CRY-14 in myocardial ischemia and hy-poxia.Methods The effects of peptide CRY-14 on the proliferation and oxidative stress of H9C2 myocardial cells after ischemia and hypoxia was analyzed.The effects of peptide CRY-14 on cardiac function(LVEF,LVFS,LVEDD,LVESD)were compared after myocardial infarction in mice by M-type small animal heart ultrasound.HE staining,Tunel staining and Masson staining were used to evaluate the changes of cardiac histology and apoptosis after the intervention of CRY-14.In addition,Western Blot assay was used to investigate the mechanism of myocardial protection of peptide CRY-14.Results At the cellular level,CRY-14 alleviated the inhibitory effect of ischemia and hypoxia on the proliferation of H9C2 cardiomyocytes,and reduced the oxidative stress response caused by ischemia and hypoxia.In vivo,CRY-14 alle-viated the changes of cardiac function after myocardial infarction,increase LVEF and LVFS,and decrease LVEDD and LVESD.CRY-14 improved significantly myocardial injury,reduced myocardial cell apoptosis and myocardial fibrosis in mice after myocardial infarction.In addition,Western blot results suggested that CRY-14 can downregulate the expression of apoptosis related protein Caspase 3 and PARP in H9C2 cardiomyocytes after ischemia and hypoxia,while CRY-14 up-regulated the expression of HSP70 in H9C2 cardiomyocytes after ischemia and hypoxia.Conclusions Peptide CRY-14 plays a myocardial protective role by improving oxidative stress and apoptosis levels in ischemic heart disease,and its mechanism may be related to the regulation of HSP70.关键词
心肌缺血/低氧/肽类/肌细胞,心脏/细胞增殖/模型,动物Key words
Myocardial ischemia/Hypoxia/Peptides/Myocytes,cardiac/Cell proliferation/Models,animal引用本文复制引用
丁晶晶,冯宪真,周军,沈啸翼,陆红,徐仲卿..多肽CRY-14保护心肌对抗缺血缺氧损伤的作用机制[J].中国临床保健杂志,2024,27(1):106-111,6.基金项目
上海市卫生健康系统重点扶持学科项目(2023ZDFC0403) （2023ZDFC0403）
上海市长宁区科学技术委员会项目(CNKW2020Y06) （CNKW2020Y06）
上海市加强公共卫生体系建设三年行动计划项目(GWV1-11.1-29) （GWV1-11.1-29）
