辽宁大学学报（自然科学版）2024，Vol.51Issue(1)：8-15,8.
HPLC及LC-MS测定榛子中氯吡苯脲和多菌灵残留
Determination of Chlorfenuron and Carbendazim in Hazelnut by HPLC and LC-MS
摘要
Abstract
In this study,high performance liquid chromatography(HPLC)and liquid chromatography-mass spectrometry(LC-MS)are used to determine the residual content of chlorfenuron and carbendazim in dried hazelnuts.Both HPLC and LC-MS use C18 chromatographic columns,and the content determination adopts isocratic elution.Mobile phase:methanol-water(55∶45 by volume);volume flow:1.0 mL·min-1;measurement wavelength:260 nm;column temperature:30℃.LC-MS uses electrospray ion source,gradient elution,volume flow rate:0.6 mL·min-1,column temperature:30℃.The results show that there are residues of chlorfenuron and carbendazim in hazelnuts.The content of chlorfenuron hand good linearity in the range of 1.00～10.00 μg·mL-1.The sample recovery rate is 95.58%～100.58%;the content of carbendazim is 1.005～15.075 μg·mL-1,and the sample recovery rate is 95.61%～104.39%.Experiments have proved that the establishment of the method combining HPLC with LC-MS has the advantages of simple operation,high sensitivity and low detection limit,and can effectively detect the bulking agent chlorfenuron and more fungicides in hazelnut samples.The residues of bacteriostat were determined,and the results of linearity and recovery were satisfactory.According to the two pesticide residues of chlorfenuron and carbendazim in the 8 batches of samples tested,it is inferred that hazelnuts are safe to eat as general dried fruits.关键词
榛子/氯吡苯脲/多菌灵/高效液相色谱法(HPLC)/液相色谱-质谱法(LC-MS)Key words
hazelnut/chlorfenuron/carbendazim/high performance liquid chromatography(HPLC)/liquid chromatography-mass spectrometry(LC-MS)分类
化学化工引用本文复制引用
桑育黎,王沛,郝延军,李楠楠,戚建忠..HPLC及LC-MS测定榛子中氯吡苯脲和多菌灵残留[J].辽宁大学学报（自然科学版）,2024,51(1):8-15,8.基金项目
辽宁大学大学生创新创业训练计划项目(D201911231532324418,D2019911231644210910) （D201911231532324418,D2019911231644210910）
