中南医学科学杂志2024，Vol.52Issue(2)：163-166,4.DOI:10.15972/j.cnki.43-1509/r.2024.02.002
大蒜素改善PM2.5暴露诱导的HUVEC自噬损伤
Allicin improves HUVEC autophagic damage induced by PM2.5 exposure
摘要
Abstract
Aim To investigate the mechanism of allicin on PM2.5-induced autophagy in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were divided into control group,PM2.5 group(5 mg/L),allicin group(10 mg/L)and alli-cin combined group(10 mg/L allicin and5 mg/L PM2.5).The number of autophagosomes in each group was observed by transmis-sion electron microscopy.The needle count of LC3,p62 and the protein expressions of p62,p38,Beclin 1,LC3BⅡ/Ⅰand the phos-phorylation level of p38 were detected by cell immunofluorescence and Western blotting.Results Compared with the control group,the cell viability of PM2.5 group was significantly decreased,whereas the number of autophagosomes was increased and the protein expres-sions of Beclin 1,LC3BⅡ/Ⅰand p62 were increased(P<0.05).Compared with the PM2.5 group,the cell viability of allicin combined group was increased,the number of autophagosomes was decreased,and the protein levels of Beclin 1,LC3BⅡ/Ⅰand p62,as well as the phosphorylation level of p38 were decreased(P<0.05).Conclusion Allicin improves PM2.5-induced HUVEC injury,which may be related with the inhibition of phosphorylation of p38 protein and promotion of autophagy flow inhibited by PM2.5.关键词
大蒜素/PM2.5/人脐静脉内皮细胞/自噬Key words
allicin/PM2.5/human umbilical vein endothelial cells/autophagy分类
医药卫生引用本文复制引用
陈舒婷,刘远凤,张瑛,曹运长,封少龙..大蒜素改善PM2.5暴露诱导的HUVEC自噬损伤[J].中南医学科学杂志,2024,52(2):163-166,4.基金项目
国家自然科学基金(41877390) （41877390）
有机地球化学国家重点实验室开放课题(SKLOG202213) （SKLOG202213）
