青岛大学学报（医学版）2024，Vol.60Issue(1)：6-11,6.DOI:10.11712/jms.2096-5532.2024.60.014
纯钛表面负载无定形多聚磷酸钙涂层对成骨分化影响
Effect of pure titanium surface-loaded amorphous calcium polyphosphate coating on osteogenic differentiation
摘要
Abstract
Objective To investigate the effect of pure titanium surface-loaded amorphous calcium polyphosphate(Ca-polyP)coating on osteogenic differentiation.Methods TA2 titanium sheets were taken,coated with Ca-polyP on the surface,and characterized by field emission scanning electron microscopy,X-ray energy dispersive spectroscopy,X-ray diffractometry,Fou-rier transform infrared spectroscopy,and contact angle measurement.Mouse embryo osteoblast precursor cells were inoculated on pure titanium sheets(control group)and titanium sheets loaded with Ca-polyP coating(experimental group)for cell culture.The proliferation activity of cells was measured by CCK-8 assay.The osteogenic differentiation ability of cells on the surface of titanium sheets in both groups was assessed by alkaline phosphatase activity assay and alkaline phosphatase staining.Results Successful loading of 20-chain-length Ca-polyP coating on titanium sheet surface was demonstrated by various physical characterization me-thods.The results of CCK-8 assay showed that titanium sheets in both groups were non-cytotoxic,and the cell proliferation activity on the surface of titanium sheets in the experimental group was significantly higher than that in the control group after 7 days of co-culture(F=1 375.183,P＜0.001).With the extension of co-culture time,the alkaline phosphatase activity of cells on the surface of titanium sheets gradually increased in both groups,and the alkaline phosphatase activity of cells on the surface of titanium sheets in the experimental group was significantly higher than that in the control group after 7 days of co-culture(F=41.141,P＜0.01).Al-kaline phosphatase staining followed by inverted fluorescence mi-croscopy showed that there were significantly more blue-purple dark-ly stained nodules on titanium sheets in the experimental group than in the control group.Conclusion Titanium sheets loaded with Ca-polyP coating on the surface significantly enhances the proliferation and osteogenic differentiation of mouse embryo osteoblast precursor cells compared with pure titanium sheets.关键词
钛/磷酸钙类/小鼠胚胎干细胞/成骨分化Key words
titanium/calcium phosphates/mouse embryonic stem cells/osteogenic differentiation分类
医药卫生引用本文复制引用
单烁,周欢,陈胜男,魏子然,杨磊,于腾波..纯钛表面负载无定形多聚磷酸钙涂层对成骨分化影响[J].青岛大学学报（医学版）,2024,60(1):6-11,6.基金项目
国家自然科学基金杰出青年基金(82025025) （82025025）
国家自然科学基金青年科学基金(31802022) （31802022）
山东省重点研发计划项目(2021SFGC0502) （2021SFGC0502）
河北省自然基金绿色通道项目(C2021-202003) （C2021-202003）
河北省自然基金创新研究群体项目(H2022202007) （H2022202007）
天津市多元投入基金项目面上项目(21JCYBJC01030) （21JCYBJC01030）
国家骨科与运动康复临床医学研究中心创新基金项目(2021-NCRC-CXJJ-Z-H-17) （2021-NCRC-CXJJ-Z-H-17）
