青岛大学学报（医学版）2024，Vol.60Issue(1)：12-16,5.DOI:10.11712/jms.2096-5532.2024.60.004
右美托咪定对神经元氧糖剥夺/复氧损伤的保护作用
Protective effects of dexmedetomidine against neuronal oxygen-glucose deprivation/reoxygenation injury
摘要
Abstract
Objective To investigate the neuroprotective effects of dexmedetomidine(Dex)against oxygen-glucose depriva-tion/reoxygenation(OGD/R)injury of cortical neurons and its possible mechanism of action.Methods The cortical neurons of fetal Sprague-Dawley rats(gestational age,18 d)were cultured and then were randomly divided into sham group(cultured in a sugar-containing medium in normal incubator),OGD/R group(cultured in a sugar-free medium in an anaerobic incubator at 37 ℃to prepare a neuronal OGD/R injury model),OGD/R+LW6 group(OGD/R neurons treated with 1 mmol/L LW6 for 1 h as a po-sitive control),OGD/R+low-concentration Dex group(OGD/R neurons treated with 0.1 μmol/L Dex for 1 h),and OGD/R+high-concentration Dex group(OGD/R neurons treated with 1.0 μmol/L Dex for 1 h).The survival rate of cells was determined by co-lorimetric assay with Cell Counting Kit-8(CCK-8)and immunofluorescence assay.The expression levels of hypoxia-inducible factor-1α(HIF-1α),activating transcription factor 6(ATF-6),and the downstream target C/EBP homologous protein(CHOP)were mea-sured by Western blot.Results According to CCK-8 and immunofluorescence assay results,there was a significant diffe-rence in the survival rate of neurons between the groups(F=63.46,P＜0.05).Pairwise comparison showed that the survival rate in the OGD/R group was significantly lower than that in the sham group(P＜0.05);and the survival rate of the OGD/R+high-con-centration Dex group was significantly increased compared with that of the OGD/R group(P＜0.05).Western blot results showed significant differences in the expression of HIF-1α,ATF-6,and CHOP proteins(F=80.30-155.36,P＜0.05).Pairwise compari-son showed that the expression of HIF-1α,ATF-6,and CHOP was significantly higher in the OGD/R group than in the sham group(P＜0.05);and the OGD/R+high-concentration Dex group showed significantly lower expression of HIF-1α,ATF-6,and CHOP compared with the OGD/R group,the differences are statistically significant(P＜0.05).Conclusion Dex inhibits HIF-1α expression to exert protective effects in the neuronal model of OGD/R injury.关键词
右美托咪定/低氧诱导因子1α/氧糖剥夺/复氧损伤/激活转录因子6/神经保护Key words
dexmedetomidine/hypoxia-inducible factor-1alpha/oxygen glucose deprivation/reoxygenation injury/activating transcription factor 6/neuroprotection分类
医药卫生引用本文复制引用
张雅瑞,侯庆明..右美托咪定对神经元氧糖剥夺/复氧损伤的保护作用[J].青岛大学学报（医学版）,2024,60(1):12-16,5.基金项目
国家自然科学基金资助项目(8217051911) （8217051911）
