青岛大学学报（医学版）2024，Vol.60Issue(1)：28-32,5.DOI:10.11712/jms.2096-5532.2024.60.017
白及多糖在小鼠烟曲霉菌性角膜炎中的抗炎作用
Anti-inflammatory effect of Bletilla striata polysaccharides in mice with Aspergillus fumigatus keratitis
摘要
Abstract
Objective To investigate the anti-inflammatory mechanism of Bletilla striata polysaccharides(BSP)in a mouse model of Aspergillus fumigatus keratitis and the therapeutic effect of BSP on fungal keratitis.Methods A mouse model of fun-gal keratitis(FK)was established,and BSP solution was used for local treatment as eye drops.A slit lamp was used to observe the severity of keratitis on days 1,3,and 5 after infection;RT-PCR was used to measure the mRNA expression levels of inflammatory cytokines and the pattern recognition receptor Dectin-1 in mouse cornea,and ELISA and Western blotting were used to measure the protein expression levels inflammatory cytokines and Dectin-1.Results The BSP group had a significantly lower clinical score of corneal inflammation than the control group on days 3 and 5 after infection(F=6.64,11.58,P＜0.05).BSP solution at a concentration of 16 g/L significantly reduced the mRNA and protein expression levels of the inflammatory cytokines interleukin-1p,interleukin 6,and tumor necrosis factor-α and the pattern recognition receptor Dectin-1 in mouse cornea(F=2.21-97.39,P＜0.05).Conclusion BSP exerts an anti-inflammatory effect in mice with A.fumigatus keratitis by downregulating the expression levels of inflammatory cytokines and inhibiting the expression of the pattern recognition receptor Dectin-1.关键词
白及多糖/烟曲霉菌/角膜炎/炎症/小鼠Key words
Bletilla striata polysaccharide/Aspergillus fumigatus/keratitis/inflammation/mice分类
医药卫生引用本文复制引用
张冉冉,彭旭东,赵桂秋..白及多糖在小鼠烟曲霉菌性角膜炎中的抗炎作用[J].青岛大学学报（医学版）,2024,60(1):28-32,5.基金项目
国家自然科学基金资助项目(81870632) （81870632）
山东省自然科学基金青年计划(ZR2019BH004) （ZR2019BH004）
