青岛大学学报（医学版）2024，Vol.60Issue(1)：67-71,5.DOI:10.11712/jms.2096-5532.2024.60.027
EBV相关肿瘤和健康人群BALF2基因序列分析
Sequence analysis of BALF2 gene in people with EBV-associated tumors and healthy people
摘要
Abstract
Objective To investigate the polymorphism of the BALF2 gene and its distribution characteristics and its rela-tionship with the development and progression of Epstein-Barr virus(EBV)-associated tumors.Methods DNA sequence analysis was performed on 349 EBV-positive specimens using nested PCR and DNA sequencing.Lasergene software was used for sequence alignment of them with the standard EBV strain B95-8 to derive a phylogenetic tree for classification of gene variants.Results According to the phylogenetic tree,there were five BALF2 genotypes:BALF2-A,BALF2-B,BALF2-C,BALF2-D,and BALF2-E.The detection rate of BALF2-E was higher in people with nasopharyngeal carcinoma than in healthy people,and higher in southern people with nasopharyngeal carcinoma than in northern people with this disease in China(x2=10.26,P＜0.01).Con-clusion The BALF2 gene is polymorphic,and the distribution of its variants is associated with tumor types and geographic re-gions.The BALF2-E variant is related to the development of nasopharyngeal carcinoma,especially in the high-incidence areas.关键词
疱疹病毒4型,人/多态性,单核苷酸/淋巴瘤/胃肿瘤/鼻咽癌Key words
herpesvirus 4,human/polymorphism,single nucleotide/lymphoma/stomach neoplasms/nasopharyngeal car-cinoma分类
医药卫生引用本文复制引用
于彩霞,赵梦鹤,肖华,王云,刘雯..EBV相关肿瘤和健康人群BALF2基因序列分析[J].青岛大学学报（医学版）,2024,60(1):67-71,5.基金项目
山东省自然科学基金资助项目(ZR2020MH302) （ZR2020MH302）
