四川中医2024，Vol.42Issue(1)：70-75,6.
少腹逐瘀汤减少E2、PGE2合成抑制内异症模型大鼠异位病灶的机制研究
Shaofu Zhuyu Decoction prevents the progression of endometriosis in a rat model by attenuating estrogen and pros-taglandin E2 generation
摘要
Abstract
Objctive The aim of this study was to determine the mechanism of Shafu Zhuyu Decoction(SFZYD)in a rat en-dometriosis model.Methods Autologous uterine transplantation was performed in Sprague Dawley(SD)rats,and those with suc-cessful ectopic implants were randomly divided into model group,letrozole group and SFZY group,and were treated with saline placebo,letrozole or SFZYD for a period of 4weeks.The implant volume was recorded before and after medication,and serum sex hormone levels as well as the levels of estrogen and prostaglandin(PG)E2 in the ectopic lesions were determined by ELISA.The expression of COX-2,StAR,aromatase,ERα and ERβ in the lesions was detected by immunohistochemistry and Western blot-ting.Results Both letrozole and SFZYD reduced the size of endometriosis lesions.Letrozole decreased E2 and E1 levels in both ser-um and lesions,increased FSH and LH levels in serum;SFZYD only targeted E2 and E1 levels in the lesions and increased FSH in serum.Both drugs reduced local COX-2expression and inhibited PGE2 production without affecting StAR and aromatase expres-sion.In addition,letrozole upregulated ERα with no significant impact on ERβ,whereas SFZYD not only up-regulated ERα but also down-regulated ERβ.Conclusion The mechanism of Shaofu Zhuyu Decoction on endometriosis mice may be realized by re-ducing E2 synthesis in the ectopic lesions by lowering COX-2expression and inhibiting PGE2 production,along with balancing ERα/ERβ expression ratio.关键词
少腹逐瘀汤/子宫内膜异位症/局部雌激素/炎症Key words
Shaofu Zhuyu decoction/endometriosis/local estrogen/inflammation分类
医药卫生引用本文复制引用
曹颖,赵舒,江春花,徐丁洁..少腹逐瘀汤减少E2、PGE2合成抑制内异症模型大鼠异位病灶的机制研究[J].四川中医,2024,42(1):70-75,6.基金项目
河北省自然科学基金(编号:H2020209123) （编号:H2020209123）
国家自然科学基金(编号:81603455). （编号:81603455）
