四川中医2024，Vol.42Issue(1)：80-83,4.
基于"脑肠轴理论"探讨龟羚帕安丸对PD大鼠纹状体DA及血清CCK、VIP水平影响的研究
Based on the Theory of Brain-gut Axis,to Explore the Effects of Guiling Pa'an Pill on Striatal DA and Serum CCK and VIP Levels in PD Rats
摘要
Abstract
Objective Based on the brain-gut axis theory,the effects of Guilingpa'an Pill on the levels of dopamine(Dopa-mine,DA)in striatum lysate and serum cholecystokinin(cholecystokinin,CCK)and vasoactive intestinal peptide(vasoactive intestinal peptide,VIP)in PD rats were studied.Methods The PD rat model was made by injection of 6-hydroxydopamine(6-OHDA).The successful PD rats were randomly divided into model group(n=15),western medicine group(n=15),high dose group(n=15),middle dose group(n=15)and low dose group(n=15).The model group and sham operation group were given the same volume of normal saline,and the other groups were given corresponding drugs for 4weeks.Blood samples were taken from abdominal aorta of rats,and the levels of DA in striatum lysate and serum CCK and VIP were detected by enzyme-linked im-munosorbent assay(ELISA).Results Compared with sham operation group,the levels of striatum DA,serum CCK and VIP in model group,western medicine group and each dose group were significantly lower than those in sham operation group(P＜0.01);Compared with the model group,the levels of striatum DA,serum CCK and VIP in the western medicine group and each dose group were significantly higher than those in the model group(P＜0.01).Conclusion Guiling Pa'an Pill has obvious neuro-protective effect,and its mechanism is related to activating brain-gut axis,increasing the level of CCK and VIP,increasing the level of DA in substantia nigra and striatum of midbrain,and improving intestinal function.关键词
帕金森病/龟羚帕安丸/多巴胺/胆囊收缩素/血管活性肠肽Key words
Parkinson's disease/Guiling Pa'an pill/DA/CCK/VIP分类
医药卫生引用本文复制引用
常学辉,张良芝,陈帅杰,王冬莲,张创业..基于"脑肠轴理论"探讨龟羚帕安丸对PD大鼠纹状体DA及血清CCK、VIP水平影响的研究[J].四川中医,2024,42(1):80-83,4.基金项目
河南省科技攻关项目(编号:222102310630) （编号:222102310630）
河南省中医药科学研究专项课题(编号:2019ZYBJ16) （编号:2019ZYBJ16）
河南省中医药拔尖人才培养项目(豫中医科教[2018]35号) （豫中医科教[2018]35号）
全国名老中医药专家传承工作室建设项目(豫卫中医函(2022)3号). （豫卫中医函(2022）
