TMEM206基因敲除小鼠小脑组织中差异表达基因的筛选及生物学功能分析OACSTPCD
Screening of differentially expressed genes in cerebellar tissues of TMEM206-knockout mice and analysis of their biological functions
目的 筛选TMEM206基因敲除小鼠小脑组织中的差异表达基因并进行生物学功能分析,探讨TMEM206基因的功能.方法 采用CRISPR/Cas9基因编辑技术制备TMEM206基因敲除小鼠,并通过配殖获得TMEM206基因敲除纯合小鼠.选取3只8周龄雌性TMEM206基因敲除纯合小鼠和3只同窝雌性野生小鼠,分离小脑组织并提取总RNA,转录组测序后,使用Cuffdiff(v2.2.1)软件的EBseq算法筛选差异表达基因,并使用DAVID数据库对差异…查看全部>>
Objective To screen the differentially expressed genes in the cerebellar tissues of TMEM206-knockout mice and to analyze their biological functions to explore the function of the TMEM206 gene.Methods TMEM206-knock-out mice were generated via the CRISPR-Cas9 system.The homozygous TMEM206-knockout mice were obtained by mat-ing.Total RNAs were extracted from cerebellum in three 30-week-old homozygous TMEM206-knockout female mice and three wild-type female litte…查看全部>>
陆盈;孙顺昌
上海交通大学医学院附属瑞金医院检验科,上海 200025上海交通大学医学院附属瑞金医院检验科,上海 200025||上海交通大学医学院附属瑞金医院无锡分院检验科
临床医学
TMEM206基因小脑组织转录组测序
TMEM206 genecerebellumtranscriptome sequencing
《山东医药》 2024 (9)
腓骨肌萎缩症新致病基因的鉴定及功能研究
10-13,4
国家自然科学基金项目(31571294).
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