山东医药2024，Vol.64Issue(9)：19-23,5.DOI:10.3969/j.issn.1002-266X.2024.09.005
三氯化铝诱发阿尔茨海默病过程中熊果酸灌胃对大鼠认知功能的改善作用观察
Improvement effect of ursolic acid on cognitive function in rats during the process of AlCl3-induced Alzheimer's disease
摘要
Abstract
Objective To observe the improvement effect of intragastric administration of ursolic acid(UA)on cog-nitive function in rats during the process of Alzheimer's disease(AD)induced by aluminium trichloride(AlCl3)and to ex-plore its mechanism.Methods Twenty-four male Wistar rats were randomly divided into the blank group(n=6),UA group(n=6),AlCl3 group(n=6)and AlCl3+UA group(n=6),respectively.The rats in the blank group were given normal saline,the rats in the UA group were intragastrically administered AlCl3 to establish the AD models,and the rats in the Al-Cl3+UA group were intragastrically given UA during the process of AD induced by AlCl3.After stopping intragastric admin-istration for 5 d,the cognitive function of rats in each group was observed by Morris water maze test.The rats in each group were killed by decapitation after anesthesia,and the cerebral cortex and hippocampus were taken.The aluminum content in the cerebral cortex and hippocampus was determined by spectrophotometry,and the activity of acetylcholinase(AChE)in the cerebral cortex and hippocampus was detected by colorimetry.The activity of superoxide dismutase(SOD),catalase(CAT)and glutathione(GSH)in cerebral cortex and hippocampus was measured,and the activity of thiobarbituric acid re-actants(TBARS)was calculated.The relative expression levels of inflammatory factors TNF-α,IL-6,NF-κB and COX-2 mRNA in cerebral cortex and hippocampus were detected by real-time fluorescence quantitative PCR.Results Com-pared with the blank group and the UA group,the escape latency was longer and the stay time in the original platform quad-rant was shorter in the AlCl3 group(both P<0.05).Compared with the AlCl3 group,the escape latency was shorter and the stay time in the original platform quadrant was longer in the AlCl3+UA group(both P<0.05).The content of aluminum in cerebral cortex and hippocampus in the AlCl3 group was higher than that in the other groups(P<0.05).The activity of AChE in cerebral cortex and hippocampus in the AlCl3 group was higher than that in the other groups(P<0.05).The activ-ity of TBARS in cerebral cortex and hippocampus of AlCl3 group was higher than that of the other groups,while the activity of SOD,CAT and GSH was lower than that of the other groups(all P<0.05).The relative expression levels of inflammato-ry cytokines TNF-α,IL-6,NF-κB and COX-2 mRNA in cerebral cortex and hippocampus in the AlCl3 group were higher than those in the other groups(all P<0.05).Conclusion UA can improve the cognitive function of AlCl3-induced AD model rats,and its mechanism may be related to the decrease of aluminum content,AChE activity,oxidative stress level and the expression of inflammatory factors in cerebral cortex and hippocampus.关键词
熊果酸/阿尔茨海默病/三氯化铝/乙酰胆碱酯酶/氧化应激/神经炎症/动物实验Key words
ursolic acid/Alzheimer's disease/aluminium trichloride/acetylcholinesterase/oxidative stress/neuroinflammation/animal experiment分类
医药卫生引用本文复制引用
刘萍萍,肖一,张翼,何学芳,彭红,季一飞..三氯化铝诱发阿尔茨海默病过程中熊果酸灌胃对大鼠认知功能的改善作用观察[J].山东医药,2024,64(9):19-23,5.基金项目
国家自然科学基金资助项目(81870966) （81870966）
四川省自然科学基金项目(2022NSFSC0756). （2022NSFSC0756）
