山东医药2024，Vol.64Issue(9)：47-51,5.DOI:10.3969/j.issn.1002-266X.2024.09.011
姜黄素灌胃对脂肪肝大鼠肠黏膜屏障损伤的改善作用及其机制
Improvement effect of curcumin on intestinal mucosal barrier injury rats with fatty liver
摘要
Abstract
Objective To observe the improvement effect of the intragastric administration of curcumin on intestinal mucosal barrier injury rats with fatty liver,and to explore its mechanism of action.Methods Non-alcoholic fatty liver disease rat models were established by high-fat diet,and then were randomly divided into the high fat group,low-dose cur-cumin group,and high-dose curcumin group,with 10 mice in each group.Anther 10 normal rats were used as the control group.Rats in low-dose curcumin group were given curcumin 200 mg/(kg·d)by gavage,while rats in the high-dose group were given curcumin 400 mg/(kg·d)by gavage.Rats in the control and model groups were given sodium carboxy-methyl cellulose by gavage,and rats in each group were sacrificed after 8 weeks of gavage.We took the liver and small in-testine tissues from rats of each group,and observed pathological changes under light microscopy by HE staining.We took the portal vein blood from rats and measured LPS,diamine oxidase(DAO),ALT and AST.The small intestine tissues were taken to measure the content of malondialdehyde(MDA)and the activity of superoxide dismutase(SOD).Western blotting was used to detect nuclear factor E2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)proteins in the small in-testine tissues.Results Hepatocyte architecture was normal in the control group.Lipid droplets of varying sizes were seen in hepatocytes of the model group.Hepatocellular steatosis was attenuated in the low-dose group and high-dose group.The villi of rats in the control group showed no congestion and edema,while the villi of rats in the model group showed structural damage.The villi of rats in the low-dose and high-dose curcumin groups showed loss and reduction.Compared with the control group,the blood levels of DAO,LPS,AST,and ALT in the model group,low-dose curcumin group,and high-dose curcumin group increased(all P<0.05).However,those in the low-dose and high-dose curcumin groups were lower than those of the model group(all P<0.05),with a dose-dependent manner(all P<0.05).Compared with the control group,MDA content of intestine in the model group,low-dose curcumin group,and high-dose curcumin group increased(P<0.05),but that in the low-dose and high-dose curcumin groups was lower than that of the model group,with a dose-dependent manner(P<0.05).Compared with the control group,SOD activity of intestine in the model group,low-dose curcumin group,and high-dose curcumin group decreased(P<0.05),but that in low-dose curcumin group and high-dose curcumin group was higher than model group,with a dose-dependent manner(P<0.05).Compared with the control group,the SOD activity of small intestine tissues in the model group,low-dose curcumin group,and high-dose curcumin group decreased(all P<0.05),but that in the low-dose curcumin group and high-dose curcumin group was higher than that in the model group(all P<0.05),with a dose-dependent manner(all P<0.05).Compared with the con-trol group,the relative expression levels of Nrf2 and HO-1 proteins in the small intestine tissues of the model group,low-dose curcumin group,and high-dose curcumin group increased(all P<0.05),and those were higher in the low-dose cur-cumin group and high-dose curcumin group than in the model group(all P<0.05),and there was in a dose-dependent man-ner(all P<0.05).Conclusion Intragastric administration of curcumin can alleviate intestinal mucosal barrier damage in rats with fatty liver,and its mechanism of action may be related to activating the Nrf2/HO-1 pathway to improve intesti-nal oxidative stress status.关键词
姜黄素/脂肪肝/肠黏膜屏障/氧化应激/核因子-E2相关因子2/血红素加氧酶-1Key words
curcumin/fatty liver/intestinal mucosal barrier/oxidative stress/nuclear factor E2-related factor 2/heme oxygenase-1分类
医药卫生引用本文复制引用
侯洪涛,张建,裘艳梅,李苹苹,郑吉敏,王玉珍,刘改芳..姜黄素灌胃对脂肪肝大鼠肠黏膜屏障损伤的改善作用及其机制[J].山东医药,2024,64(9):47-51,5.基金项目
河北省医学科学研究重点课题计划(20210710). （20210710）
