山东医药2024，Vol.64Issue(10)：16-20,5.DOI:10.3969/j.issn.1002-266X.2024.10.004
lncR-HOXA-AS3靶向miR-29a对前列腺癌细胞凋亡和自噬的影响
LncR-HOXA-AS3 targeting miR-29a affects apoptosis and autophagy of prostate cancer cells
摘要
Abstract
Objective To investigate the effects of long chain non-coding RNA(lncR)HOXA-AS3 and microRNA-29a(miR-29a)on apoptosis and autophagy of prostate cancer(PCa)cells.Methods Human PCa cells(DU-145,LN-CaP,C4-2B)and human normal prostate cells RWPE-1 were cultured in vitro,and RT-qPCR was used to detect the rela-tive expression of lncR-HOXA-AS3 and miR-29a in the cells.Dual luciferase reporter assay was used to verify the targeted regulatory relationship between lncR-HOXA-AS3 and miR-29a.LNCaP cells in the logarithmic growth phase were divided into the si-NC group,si-HOXA-AS3 group,si-HOXA-AS3+anti-miR-NC group and si-HOXA-AS3+ anti-miR-29a group.Si-NC,si-HOXA-AS3,si-HOXA-AS3+anti-miR-NC,si-HOXA-AS3+ anti-miR-29a were transfected into LNCaP cells of the above groups by liposome transfection.The expression changes of lncR-HOXA-AS3 and miR-29a in each group were detected by RT-qPCR,and the apoptosis of cells in each group was detected by flow cytometry,and the expression levels of apoptosis-related proteins Caspase-3,autophagy related proteins LC3Ⅱ,LC3Ⅰ and Beclin 1 in each group were detected by Western blotting.Results Compared with the normal prostate cells,the expression of lncR-HOXA-AS3 significantly increased(P<0.05),while the expression of miR-29a significantly decreased in human PCa cells(P<0.05).Dual lucifer-ase reporter genes showed that lncR-HOXA-AS3 inhibited luciferase activity by targeting miR-29a(P<0.05).Compared with the si-NC group,the lncR-HOXA-AS3 expression of LNCaP cells decreased,and the miR-29a expression increased(both P<0.05),the apoptosis rate significantly increased(P<0.05),and the protein expression levels of Caspase-3,LC3Ⅱ/LC3Ⅰ and Beclin 1 significantly increased in the si-HOXA-AS3 group(all P<0.05).Compared with the si-HOXA-AS3+anti-miR-NC group,the expression of miR-29a significantly decreased(P<0.05),the apoptosis rate significantly de-creased(P<0.05),and the expression levels of Caspase-3,LC3Ⅱ/LC3Ⅰ,and Beclin 1 proteins significantly decreased in the si-HOXA-AS3+anti-miR-29a group(all P<0.05).Conclusion Inhibiting lncR-HOXA-AS3 can target and regulate the miR-29a gene to promote apoptosis and autophagy in PCa cells.关键词
前列腺癌/长链非编码RNA-HOXA-AS3/微小RNA-29a/细胞凋亡/细胞自噬Key words
prostate carcinoma/long non-coding RNA-HOXA-AS3/miR-29a/apoptosis/autophagy分类
医药卫生引用本文复制引用
李强,刘晶,张国民,王亮,刘志飞,朱研峰..lncR-HOXA-AS3靶向miR-29a对前列腺癌细胞凋亡和自噬的影响[J].山东医药,2024,64(10):16-20,5.基金项目
河北省医学科学研究课题(20220216). （20220216）
