山东医药2024，Vol.64Issue(10)：36-40,5.DOI:10.3969/j.issn.1002-266X.2024.10.008
当归芍药散含药血清及其有效成分对肾间质纤维化大鼠的作用及机制
Effects of Danggui Shaoyao San-medicated serum and its active ingredients on renal interstitial fibrosis rats
摘要
Abstract
Objective To investigate the interventional effects of Danggui Shaoyao San(DSS)-medicated serum and its active ingredients ferulic acid(FA)and paeoniflorin(PF)on renal interstitial fibrosis(RIF)rats and the mecha-nism.Methods DSS solution was prepared and administered to male Sprague Dawley rats by gavage at the same time of the day at low(0.05 mL DSS solution/10 g body mass),medium(0.1 mL DSS solution/10 g body mass),and high(0.2 mL DSS solution/10 g body mass)doses for 7 d.Blood was obtained from the abdominal aorta to obtain the equiva-lent low-,medium-,and high-dose of DSS serum(DSSS).Rat renal tubular epithelial cells(NRK-52E)were divided in-to the control group(Control),the model group(treated with TGF-β),the siRNA transfection group(treated with TGF-β +siRNA),the FA group(treated with TGF-β+FA),the PF group(treated with TGF-β+PF),and the low-,medium-,and high-dose DSSS groups(treated with TGF-β+DSS-L,TGF β+DSS-M,and TGF-β+DSS-H),respectively.The inter-vention concentrations of DSSS,FA,PF and TGF-β on NRK-52E cells were screened and determined for NRK-52E cells in the above groups.Western blotting was used to detect the changes in the protein levels of NLRP3,cysteine protease 1(CASP1),Gasdermin D(GSDMD),interleukin-18(IL-18),fibronectin(FN),E-Cadherin,and N-Cadherin in each group.Results Compared with the model group,the expression levels of pyroptosis-related proteins NLRP3,CASP1,GSDMD,and IL-18 decreased in the DSSS,FA,and PF groups,and the difference was statistically significant(all P<0.05).Compared with the model group,the expression levels of fibrosis-related adhesion proteins FN,E-Cadherin and N-Cadherin decreased in the medium-dose and high-dose DSSS and FA and PF groups,and the difference was statistically significant(all P<0.05).Conclusion DSS-medicated serum and its active ingredients,paeoniflorin and ferulic acid,can inhibit the activation of NLRP3-associated pyroptosis proteins in the TGF-β-induced fibrosis model of NRK-52E cells and regulate the expression of adhesion proteins and the progression of epithelial-mesenchymal transdifferentiation to allevi-ate the fibrosis.关键词
慢性肾脏疾病/肾间质纤维化/当归芍药散/细胞焦亡/肾小管上皮细胞Key words
chronic kidney disease/renal interstitial fibrosis/Danggui Shaoyao San/pyroptosis/renal tubular ep-ithelial cells分类
医药卫生引用本文复制引用
刘化君,张挺..当归芍药散含药血清及其有效成分对肾间质纤维化大鼠的作用及机制[J].山东医药,2024,64(10):36-40,5.基金项目
上海市卫生健康委员会中医药科研项目(2016JP013). （2016JP013）
