上海针灸杂志2024，Vol.43Issue(4)：456-463,8.DOI:10.13460/j.issn.1005-0957.2024.04.0456
电针对胰岛素抵抗大鼠下丘脑自噬活性的影响
Effect of electroacupuncture on autophagy activity in the hypothalamus of insulin resistant rats
摘要
Abstract
Objective To observe the effects of electroacupuncture on the expression of autophagy regulatory genes Atg1,Atg13,Beclin 1,and glycogen syn-thase kinase-3(GSK3β)protein in the hypothalamus of insulin resistant rats and explore the potential mechanism of acupuncture in treating insulin resistance.Method Forty-five clean Wistar rats were randomly divided into a normal group(15 rats)and a modeling group(30 rats).The normal group was fed conventionally,and the modeling group was fed a high-fat diet with 10%fructose water for 8 weeks to prepare the insulin resistant rat model.After successful modeling,30 rats in the modeling group were further randomized into a model group(15 rats)and an electroacupuncture group(15 rats).Rats in the electroacupuncture group received electroacupuncture intervention.The fasting blood glucose(FBG)and fasting insulin(FINS)were detected using a micro-glucometer and enzyme-linked immunosorbent assay(ELISA),and the insulin resistance index(IRI)was calculated to evaluate the degree of insulin resistance.The mRNA and protein expression levels of Atg1,Atg13,Beclin-1,and GSK3β in the hypothalamus tissues were examined using Western blot and real-time polymerase chain reaction(PCR)to assess the effect of electroacupuncture on autophagy activity in the hypothalamus of insulin resistant rats.Result After modeling,the IRI increased markedly in the modeling group compared with the normal group(P＜0.05).After the intervention,the mRNA and protein expression levels of Atg1,Atg13,Beclin-1,and GSK3β increased in the model group compared with the normal group(P＜0.05);compared with the model group,the mRNA and protein expression levels of Atg1,Atg13,Beclin-1,and GSK3β dropped in the electroacupuncture group(P＜0.05),as well as the IRI(P＜0.05).Conclusion Electroacupuncture can significantly improve insulin resistance induced by high fat and high sugar.The mechanism may be related to inhibiting the excessive autophagy of hypothalamic cells by down-regulating the expression of autophagy-related factors Atg1,Atg13,and Beclin-1 and reducing the expression of GSK3β.关键词
针刺疗法/电针/胰岛素抵抗/血糖/自噬/下丘脑/大鼠Key words
Acupuncture therapy/Electroacupuncture/Insulin resistance/Blood glucose/Autophagy/Hypothalamus/Rats分类
医药卫生引用本文复制引用
杨鸿静,张萌,吴松,王静芝..电针对胰岛素抵抗大鼠下丘脑自噬活性的影响[J].上海针灸杂志,2024,43(4):456-463,8.基金项目
国家自然科学基金项目(81904276) （81904276）
湖北省卫生健康委员会中医药科研项目青年基金项目(ZY2021Q008) （ZY2021Q008）
针灸治未病湖北省协同创新项目(HBPCIC-2020-08) （HBPCIC-2020-08）
国家中医药管理局岐黄工程项目[国中医药人教函(2018)284号] （2018）
