摘要
Abstract
Objective To observe the concentration change of Metrnl in type 2 diabetes mice and its effect on aortic re-modeling in mice,and explore its possible mechanism.Methods Fifteen healthy male mice were randomly divided into control group(Control group),diabetes group(DM group),and Metrnl intervention group(DM + Metrnl group).The DM + Metrnl group was treated with recombinant Metrnl,while the Control and DM groups were treated with equal volumes of physiological saline.The blood lipid levels of each group of mice after injection were measured.Observation of pathological changes in mouse aorta,ROS content in the aorta,apoptosis of aortic cells,and expression levels of P-P38 MAPK,P38 MAPK,P-STAT1,STAT1,BAX,and BCL-2 proteins in the aorta was carried out.Results There was no significant difference in body mass and blood glucose between the Metrnl intervention group and the diabetes group(P＞0.05);Before intervention with recombinant Metrnl,there was no statistically significant difference in serum Metrnl concentration among the groups of mice(P＞0.05).Metrnl decreased the levels of serum TC,TG and LDL-C in type 2 diabetes mice(P＜0.05),and increased the concentration of serum HDL-C(P＜0.05);Metrnl could im-prove the morphology of aortic cells in type 2 diabetes mice,but could not change the thickness of aorta(P＞0.05);Metrnl reduced the production of reactive oxygen species,decreased cell apoptosis rate(P＜0.05),and downregulated the expression of P-P38/P38 MAPK,P-STAT1/STAT1 and BAX proteins(P＜0.05),while increasing the expression of BCL-2 protein(P＜0.05).Conclusion There is no obvious change in the level of serum Metrnl in diabetes mice.Exogenous Metrnl intervention can not improve the body weight and blood sugar of diabetes mice,but can improve the lipid metabolism of diabetes mice.Metrnl plays a protective role in aorta by inhibiting ROS/P38 MAPK/STAT1 signal pathway and alleviating oxidative stress injury.关键词
Metrnl/2型糖尿病/ROS/凋亡率/ROS/P38 MAPK/STAT1信号通路Key words
Metrnl/type 2 diabetes/ROS/apoptosis rate/ROS/P38 MAPK/STAT1 signal pathway分类
医药卫生
