生物骨科材料与临床研究2024，Vol.21Issue(2)：6-9,15,5.DOI:10.3969/j.issn.1672-5972.2024.02.002
PLK2协同p21影响骨肉瘤细胞增殖的作用机制
The mechanism of PLK2 synergistic p21 affecting the proliferation of osteosarcoma cells
陈子健 1王嘉璐 2李志钢1
作者信息
- 1. 湖北省中西医结合医院骨科,湖北 武汉,430015
- 2. 湖北中医药大学针灸骨伤学院,湖北 武汉,430061
- 折叠
摘要
Abstract
Objective To study the potential mechanism of PLK2 and p21 affecting the occurrence and development of osteosarcoma.Methods The differential expression of lncRNA in osteosarcoma tissues and normal tissues was obtained from the GEO database.HEK293 T cells and U2OS and Sao-2 human osteosarcoma cell lines were prepared for culture.The expression of PLK2 in U2OS cell lines was inhibited by siRNA technology and knockdown assay was performed.Total RNA was isolated from U2OS cells for real-time quantitative polymerase chain reaction.Immunoblotting(IB)and immunoprecipitation(IP)analysis were performed on U2OS cell lines,followed by EdU assay and cell viability assay.Finally,SPSS 17.0 version was used for statistical analysis.Cancer tissues were compared with adjacent normal tissues.Results Compared with normal tissues,PLK2 was lower in osteosarcoma tissues.In the experiment of transfecting two kinds of small interfering RNA knocking down PLK2 and recombinant plasmid carrying PLK2 into U2OS cells,it was found that the proliferation ability of U2OS cells was enhanced after PLK2 knockout,while PLK2 overexpression had the opposite effect.The in vivo interaction between PLK2 and p21 was detected in U2OS cells,and it was found that the protein level of p21 was consistent with the protein level of PLK2 and was statistically significant.Conclusion PLK2 is involved in the process of inhibiting the proliferation of osteosarcoma cell,which may occur by interacting with p21.关键词
骨肉瘤/细胞增殖/PLK2/P21/蛋白互作Key words
Osteosarcoma/Cell proliferation/PLK2/P21/Protein interaction分类
医药卫生引用本文复制引用
陈子健,王嘉璐,李志钢..PLK2协同p21影响骨肉瘤细胞增殖的作用机制[J].生物骨科材料与临床研究,2024,21(2):6-9,15,5.
