水生生物学报2024，Vol.48Issue(5)：713-724,12.DOI:10.7541/2024.2023.0238
急性氨氮胁迫下大口黑鲈的肝脏转录组特征分析
LIVER TRANSCRIPTOME OF LARGEMOUTH BASS(MICROPTERUS SAMOIDES)UNDER ACUTE AMMONIA NITROGEN STRESS
摘要
Abstract
To understand the molecular mechanism of Micropterus salmoides subjected to ammonia nitrogen stress,the HiSeq sequencing strategy of Illumina platform was used to analyze the gene expression profiles in the liver of M.salmoides at 12h ammonia nitrogen stress(12h)and 24h ammonia stress(24h).We obtained 111.66 Gb of valid data and assembled a total of 133486 unigenes with N50 value of 1000 bp.Comparative transcriptional analysis identified 2072 differentially expressed genes(DEGs)at two time points.Specifically,1516 DEGs were obtained at 12h,consist-ing of 907 up-regulated and 609 down-regulated genes,while 556 DEGs were obtained at 24h,with 330 up-regulated and 226 down-regulated.Gene ontology analysis revealed that the number of DEGs enriched at 12h was significantly higher than that at 24h,but there were more DEGs related to"Oxidoreductase activity"at 24h.In addition,KEGG enrichment analysis indicated that the DEGs were mainly involved in oxidative stress,autophagy and apoptosis related pathways.Subsequently,ten DEGs were selected for qPCR validation,and their expression levels were consistent with the sequencing analysis results,demonstrating the reliability of the sequencing data.Notably,the oxidative stress related gene Hypoxia-inducible factor 1 α(HIF1 α),Growth arrest DNA damage inducible protein 45 β(Gadd45 β),DNA damage inducible transcript 4(DDIT4),autophagy related gene microtubule-associated protein light chain 3(LC3)and apoptosis downstream related gene endoplasmic reticulum oxidoreduclin1α(Ero1 α)displayed significant upregulated throughout the entire process of ammonia nitrogen stress.In summary,ammonia nitrogen stress could cause oxidative stress response in the liver of M.salmoides,while the damaged cells were eliminated by autophagy and apoptosis when the body's regulatory role is insufficient to maintain internal environmental stability.The genes and pathways identified in this study will facilitate further investigations into the molecular mechanisms behind the tole-rance of M.salmoides to ammonia stress.关键词
氨氮胁迫/转录组/肝脏/大口黑鲈Key words
Ammonia stress/Transcriptome/Liver/Micropterus salmoides分类
农业科技引用本文复制引用
刘品,王文博,李超,严少普,李琪,罗小敏,张硕华,张艺琛,姚林荣..急性氨氮胁迫下大口黑鲈的肝脏转录组特征分析[J].水生生物学报,2024,48(5):713-724,12.基金项目
国家自然科学基金面上项目(31872175) （31872175）
陕西省自然科学基金(2021JQ-816) （2021JQ-816）
陕西省秦创原"科学家+工程师"队伍建设项目(2022KXJ-015) （2022KXJ-015）
陕西省教育厅专项(21JK0965和20JK0973)资助[Supported by the National Natural Science Foundation of China(31872175) （21JK0965和20JK0973）
Shaanxi Natural Science Foundation(2021JQ-816) （2021JQ-816）
Shaanxi Qin Chuangyuan"Scientist+Engineer"Team Construction Project(2022KXJ-015) （2022KXJ-015）
Special Project of Shaanxi Department of Education(21JK0965 and 20JK0973)] （21JK0965 and 20JK0973）
