许氏平鲉5-HT1A基因单核苷酸多态性与攻击行为表型相关性研究OA北大核心CSTPCD

We used juvenile black rockfish(Sebastes schlegelii)to investigate the impact of the 5-HT1A receptor gene on aggression control in this study.The administration of 5-HT1A receptor agonist,8-OH-DPAT,was utilized to evalua-te its impact on aggression among juvenile black rockfish.Subsequently,different aggression phenotypes within the juvenile groups were scrutinized for genetic variations in the 5-HT1A receptor gene,aiming to identify potential SNP markers correlated with aggression.Our findings showed that fish treated with 8-OH-DPAT exhibited significantly reduction in the frequency and duration of aggressive behavior compared to the control group(P＜0.05).The latency period for aggression was significantly higher in the treated group(P＜0.05).We discovered seven significant polymor-phic sites linked to attack phenotypes in the 5-HT1Aα promoter region(P＜0.05):SNP 1236,SNP 1245,SNP 1260,SNP 1301,SNP 1302,SNP 1309,and SNP 1330.Additionally,two polymorphic sites were significantly associated with attack phenotypes in the 5-HT1Aβ promoter region(P＜0.05):SNP 892 and SNP 1147.However,no significant polymorphic sites were identified in the coding region of the 5-HT1A gene in relation to attack phenotypes(P＞0.05).In summary,the results demonstrate that activating the 5-HT1A receptor effectively inhibits aggressive behavior in black rockfish.Furthermore,we also identified SNP loci within the 5-HT1A receptor gene that are associated with different aggression profiles.