水生生物学报2024，Vol.48Issue(5)：772-779,8.DOI:10.7541/2024.2023.0304
许氏平鲉5-HT1A基因单核苷酸多态性与攻击行为表型相关性研究
ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF THE 5-HT1A GENE WITH DIFFERENT AGGRESSION PHENOTYPE IN SEBASTES SCHLEGELII
摘要
Abstract
We used juvenile black rockfish(Sebastes schlegelii)to investigate the impact of the 5-HT1A receptor gene on aggression control in this study.The administration of 5-HT1A receptor agonist,8-OH-DPAT,was utilized to evalua-te its impact on aggression among juvenile black rockfish.Subsequently,different aggression phenotypes within the juvenile groups were scrutinized for genetic variations in the 5-HT1A receptor gene,aiming to identify potential SNP markers correlated with aggression.Our findings showed that fish treated with 8-OH-DPAT exhibited significantly reduction in the frequency and duration of aggressive behavior compared to the control group(P＜0.05).The latency period for aggression was significantly higher in the treated group(P＜0.05).We discovered seven significant polymor-phic sites linked to attack phenotypes in the 5-HT1Aα promoter region(P＜0.05):SNP 1236,SNP 1245,SNP 1260,SNP 1301,SNP 1302,SNP 1309,and SNP 1330.Additionally,two polymorphic sites were significantly associated with attack phenotypes in the 5-HT1Aβ promoter region(P＜0.05):SNP 892 and SNP 1147.However,no significant polymorphic sites were identified in the coding region of the 5-HT1A gene in relation to attack phenotypes(P＞0.05).In summary,the results demonstrate that activating the 5-HT1A receptor effectively inhibits aggressive behavior in black rockfish.Furthermore,we also identified SNP loci within the 5-HT1A receptor gene that are associated with different aggression profiles.关键词
单核苷酸多态性/攻击行为/5-HT1A基因/许氏平鲉Key words
Single nucleotide polymorphism/Aggressive behavior/5-HT1A gene/Sebastes schlegelii分类
生物科学引用本文复制引用
葛晓雨,李海霞,潘玉兰,陈松猛,王佳钰,马真..许氏平鲉5-HT1A基因单核苷酸多态性与攻击行为表型相关性研究[J].水生生物学报,2024,48(5):772-779,8.基金项目
广东省重点领域研发计划(2021B0202030001) （2021B0202030001）
国家自然科学基金(32102753) （32102753）
设施渔业教育部重点实验室开放课题(202215)资助[Supported by Guangdong Province Key Area Research and Development Plan Project(2021B0202030001) （202215）
National Natural Science Foundation of China(32102753) （32102753）
Open Project of Key Laboratory of Environment Controlled Aquaculture(202215)] （202215）
