护理研究2024，Vol.38Issue(7)：1139-1145,7.DOI:10.12102/j.issn.1009-6493.2024.07.002
CD41单克隆抗体建立慢性免疫性血小板减少症小鼠模型失败的初步分析
Preliminary analysis of CD41 monoclonal antibody failed to establish a mouse model of chronic immune thrombocytopenia
摘要
Abstract
Objective:CD41 monoclonal antibody(MWReg30)was injected into Balb/c mice at different times and doses to investigate whether this method can construct a stable model of chronic Immune thrombocytopenia(ITP).After the modeling,the extract of Codonopsis pilosula was used to intervene the mice,in order to clarify its adjustment effect on abnormal platelet count of model mice.Methods:Firstly,chronic ITP model was established in Balb/c mice using MWReg30,and the effect of Codonopsis pilosula's extract on the model mice was investigated after antibody injection.Subsequently,different groups of mice were given a second injection and a double dose of MWReg30 to determine whether the increase in the number of injections and dose was conducive to the establishment of chronic ITP model.Results:It was confirmed that the method of injecting MWReg30 into mice could not keep the platelet number of Balb/c mice at a low level for a long time,so the method could not establish a stable chronic ITP model,and it was difficult to achieve this purpose by increasing the number and dose of antibody injection.However,second and double dose injections of MWReg30 resulted platelet count antibody rebound to higher levels within 7 days and 14 days after the end of the injection,and the low dose of Codonopsis pilosula extract could decrease the abnormal platelet count of the model mice.Conclusion:The intraperitoneal injection of MWReg30 in mice could not reproduce stable chronic ITP models,and whether this method can be an ideal way to construct chronic ITP models remains to be further confirmed.Increasing the injection dose of MWReg30 can prolong the maintenance time of low platelet count in model mice,and low dose of Codonopsis pilosula extract may correct the abnormal elevated platelet count in model mice.关键词
慢性免疫性血小板减少症/CD41单克隆抗体/党参/模型构建/血小板计数Key words
chronic immune thrombocytopenia/CD41 monoclonal antibody/Codonopsis pilosula/model construction/platelet count引用本文复制引用
张珺,朱瑞芳,吕亚茹,李若兰,张淑文,力娜,宋凯,曾靖晖,韩世范..CD41单克隆抗体建立慢性免疫性血小板减少症小鼠模型失败的初步分析[J].护理研究,2024,38(7):1139-1145,7.基金项目
山西医科大学第一医院博士启动基金项目,编号:YB2203 （）
山西省基础研究计划项目,编号:202203021221251 （）
