特产研究2024，Vol.46Issue(2)：1-6,6.DOI:10.16720/j.cnki.tcyj.2023.060
犬细小病毒CPV-2c型亚洲株分离鉴定及VP2基因序列分析
Identification and VP2 Gene Sequence Analysis of Canine Parvovirus CPV-2c Asian Strain Isolation
摘要
Abstract
Absrtact:To investigate the unknown death of dogs in Beijing kennel.A strain of CPV was isolated from the anal swabs of dogs which suf-fering from intestinal bleeding in a Beijing dog farm.The strain was identified by PCR,hemagglutination and indirect immunofluorescence.The VP2 gene were amplified and cloned by PCR,determined its genetic branch by sequence analyse.The results showed that the isolate strain belonged to Canine parvovirus type 2c and was named CPV-BJ21 strain.The indirect immunofluorescence results of the canine mono-clonal antibody were positive.Gene sequence analysis showed that CPV-BJ21 and CPV were the same evolutionary branch,the nucleotide sequence of VP2 gene had 99％homology with cpv-2c(MH476581)in Sichuan,China;CPV-BJ21 with close genetic relationship with Asian strains.There were amino acid mutations at A5G,S297A and D426E in VP2 amino acid sequence.After 3 generations of superculture of F81 cells,the hemagglutination titer of virus solution was stable at 210.This study could provide a reference for the prevalence of CPV and the development of new vaccines.关键词
犬细小病毒/分离鉴定/VP2基因/遗传进化分析Key words
canine parvovirus/isolation and identification/VP2 gene/phylogenetic analysis分类
农业科技引用本文复制引用
李昀真,白雪,李双双,丛培强,曹海旭,鲁荣光,廉士珍,张海玲,李虹晔,胡博..犬细小病毒CPV-2c型亚洲株分离鉴定及VP2基因序列分析[J].特产研究,2024,46(2):1-6,6.基金项目
国家自然科学基金项目(32100408) （32100408）
吉林省科技发展计划项目(20210509057RQ) （20210509057RQ）
农业农村部动物病原生物学重点实验室开放课题项目(BYSWX 2021 KFKT01) （BYSWX 2021 KFKT01）
