特产研究2024，Vol.46Issue(2)：22-24,29,4.DOI:10.16720/j.cnki.tcyj.2024.040
蜜蜂资源形态测定的采样与保存方法研究
Study on Sampling and Preservation Methods for Morphological Identification of Honeybee Resources
摘要
Abstract
In order to improve the efficiency of bee morphological identification,morphological identification is an essential technique for bee germplasm evaluation.In this study,different sampling and preservation methods were used to compare the kissing rate,the amount of tissue damage,the influence of chroma and the influence of toughness of bee samples in different experimental groups.The results showed that there was no significant difference in the kissing rate of B fumigation at 20 s and 30 s in experimental groups(P＞0.05),but it was sig-nificantly higher than that in other experimental groups(P＜0.05).The number of honeybee tissue damage in experimental group D was significantly lower than that in experimental group E,F,G and control group(P＜0.05).Storage at 4 ℃ for 1 to 3 months had no effect on the chroma and toughness of honeybee sampls.It is recommended to use CO2 at the flow rate of 2 L/min for 20 s,soak in the preservation solution of 75％alcohol and 0.5％glycerol,and complete the morphological identification within 3 months.关键词
蜜蜂/采样/保存/资源鉴定/形态测定Key words
bees/sampling/preservation/morphological identification/morphological determination分类
农业科技引用本文复制引用
庄明亮,李剑飞,迟永娟,葛蓬,王志,蒋海宾,王进州,戚竞文,李志勇..蜜蜂资源形态测定的采样与保存方法研究[J].特产研究,2024,46(2):22-24,29,4.基金项目
国家重点研发计划项目(2022YFD1600200) （2022YFD1600200）
吉林省科技发展计划项目(YDZJ202301ZYTS332) （YDZJ202301ZYTS332）
国家蜂产业技术体系建设项目(CARS-44-KXJ3) （CARS-44-KXJ3）
