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貉细小病毒肠炎细胞毒株(HB-1)的分离鉴定

李卓宸 赵传芳 郭晓芹 刘昱欣 吕文雪 胡博 吕爽 张蕾 张海玲 于小亚

特产研究2024,Vol.46Issue(2):25-29,5.
特产研究2024,Vol.46Issue(2):25-29,5.DOI:10.16720/j.cnki.tcyj.2023.058

貉细小病毒肠炎细胞毒株(HB-1)的分离鉴定

Isolation and Identification of HB-1 Strain of Raccoon Dog Parvovirus Enteritis

李卓宸 1赵传芳 1郭晓芹 1刘昱欣 1吕文雪 1胡博 1吕爽 1张蕾 1张海玲 1于小亚2

作者信息

  • 1. 中国农业科学院特产研究所,吉林 长春 130112
  • 2. 吉林省科技创新平台管理中心,吉林 长春 130012
  • 折叠

摘要

Abstract

Raccoon dog parvovirus(RDPV)belongs to the family Parvoviridae and can cause parvovirus enteritis.The disease is an acute infectious disease with high morbidity and mortality,which causes huge losses to farmers and seriously restricts the healthy development of raccoon dog farming.Therefore,the development of a safer,more efficient and convenient vaccine is the trend of future research.In this stu-dy,rectal swabs of raccoon dogs with severe diarrhea were screened by CPV colloidal gold test strip,and the positive samples were cultured in F81 cells after treatment.It was identified by polymerase chain reaction detection,indirect immunofluorescence test and electron micro-scope observation.The results showed that the strain was a new strain and was named HB-1 strain.

关键词

细小病毒//分离鉴定

Key words

parvovirus/raccoon dog/separation and identification

分类

农业科技

引用本文复制引用

李卓宸,赵传芳,郭晓芹,刘昱欣,吕文雪,胡博,吕爽,张蕾,张海玲,于小亚..貉细小病毒肠炎细胞毒株(HB-1)的分离鉴定[J].特产研究,2024,46(2):25-29,5.

基金项目

吉林省科技厅重点研发项目(20200402049NC) (20200402049NC)

特产研究

OACSTPCD

1001-4721

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