天津师范大学学报（自然科学版）2024，Vol.44Issue(2)：34-41,8.DOI:10.19638/j.issn1671-1114.20240206
基于转录组的海州香薷COPT铜转运蛋白家族鉴定及响应铜胁迫表达分析
Transcriptome-based identification of copper transporter family of Elsholtzia splendens and expression analysis under copper stress
摘要
Abstract
To explore the response of COPT to copper stress in Elsholtzia splendens,bioinformatics method was used to identi-fy and analyze COPT family members in E.splendens transcriptome database.Furthermore,plant expression vector was con-structed to explore the subcellular localization of the protein.The results showed that there were 11 EsCOPT members in E.splendens transcriptome,which encode proteins with 72-159 amino acids,isoelectric point of 4.72-9.92 and the average rela-tive molecular mass of 12.870 × 103.All EsCOPTs are membrane proteins.Phylogenetic tree analysis showed that EsCOPTs belong to three subgroups.Under normal conditions,the expression of EsCOPT2 and EsCOPT9 are higher than other members.After Cu treatment,except for EsCOPT5 with unchanged expression level,all EsCOPTs showed a downregulated expression.The full-length ESCOPT9-encoded protein has three transmembrane domains and the N-terminal is short.The colocalization experi-ment showed that EsCOPT9-GFP was located on the vacuolar membrane.关键词
海州香薷/铜转运蛋白/铜胁迫/转录组Key words
Elsholtzia splendens/copper transporter/copper stress/transcriptome分类
农业科技引用本文复制引用
周仲乐,马春婕,王正宁,宋文华,马晗晗,张海燕..基于转录组的海州香薷COPT铜转运蛋白家族鉴定及响应铜胁迫表达分析[J].天津师范大学学报（自然科学版）,2024,44(2):34-41,8.基金项目
国家自然科学基金资助项目(31470292) （31470292）
天津市自然科学基金重点资助项目(18JCZDJC97900). （18JCZDJC97900）
