特产研究2024，Vol.46Issue(2)：110-114,5.DOI:10.16720/j.cnki.tcyj.2024.051
HPLC-UV法测定经典名方当归补血汤中5种化学成分含量
Determination of Five Chemical Components Content in the Classic Formula Danggui Buxue Decoction by HPLC-UV
摘要
Abstract
To establish a HPLC-UV method for the simultaneous determination of five components in Danggui buxue decoction.A diamon-sil C18 column(250 mm × 4.6 mm,5 μm)was used.The mobile phase was methanol(A):0.4％formic acid water gradient elution(B).The detection wavelength was 280 nm.Ferulic acid,SenkyunolideI,Calycosin-7-glucoside,Calycosin-7-glucoside,and Ononin had a good lin-ear relationship in the quality range of 0.091～1.824,0.015～0.308,0.092～1.848,0.036～0.728 and 0.082～1.656 μg/mL,respectively,with the correlation coefficients above 0.999.The limits of quantification were 0.091,0.015,0.092,0.036 and 0.082 μg/mL respectively;The detec-tion limits were about 0.027,0.005,0.028,0.011 and 0.025 μg/mL,respectively.The RSD of precision,repeatability,stability,and the spiked recovery test were less than 5％.The recovery rates were 99.68％,99.40％,100.24％,101.04％,and 100.88％.The method is simple,sensi-tive,rapid,and accurate.It can be used for the content determination and quality control of Danggui buxue decoction.关键词
高效液相色谱/当归补血汤/阿魏酸/洋川芎内酯Ⅰ/毛蕊异黄酮苷/毛蕊异黄酮/芒柄花苷/含量测定Key words
high performance liquid chromatography/Danggui buxue decoction/ferulic acid/senkyunolidel/calycosin-7-o-beta-D-gluco-side/calycosin/ononin/content determination分类
医药卫生引用本文复制引用
杨连升,张丽芬,陈烁,李兰霞,杨志欣..HPLC-UV法测定经典名方当归补血汤中5种化学成分含量[J].特产研究,2024,46(2):110-114,5.基金项目
黑龙江省自然科学基金项目(H2016057、H2017066) （H2016057、H2017066）
黑龙江中医药大学"优秀创新人才支持计划"项目(2012、2018) （2012、2018）
