特产研究2024，Vol.46Issue(2)：128-133,140,7.DOI:10.16720/j.cnki.tcyj.2024.052
LC-MS/MS法同时测定穿心莲中6种农药残留量
Simultaneous Determination of 6 Pesticide Residues in Andrographis paniculata by LC-MS/MS
摘要
Abstract
The aim of this study was to establish a method for the determination of 6 pesticide residues in Andrographis paniculata,so as to better control the quality of the medicinal materials.A high-performance liquid chromatography-tandem quadrupole mass spectrometer was used to test blank,sample and control samples in the scanning mode of multi-reaction monitoring(MRNI).ACQUITY UPLC BEH C18 column was used for separation,and high performance liquid chromatography-tandem mass spectrometry was used to determine the 6 pes-ticide residues commonly used in Andrographis paniculata.The results showed that the detection limits of cypermethrin,dichlorvos,2,4-drops,glyphosate,chlorpyrifos,and cypermethrin under this method were 0.015 mg/kg,0.125 mg/kg,0.04 mg/kg,0.01 mg/kg,0.03 mg/kg,and 0.025 mg/kg,respectively.Within a certain concentration range,the response values had a good linear relationship with the mass con-centration(r＞0.990),and the RSD of the six target substance reference solutions and sample spiked solutions were all less than 10％when the instrument parameters fluctuated.At room temperature,the RSD of the peak area of the six target reference solution and sample spiking solution was less than 5％within 14 hours,and the solution remained stable within 14 h.The test results of 20 batches of Andrographispan-iculata samples showed that only one batch detected 41.2 ng/g of cypermethrin,while the remaining samples were not detected,meeting the requirements.This research method is simple,linear and durable,and can be used to determine the residues of six pesticides in Andrographis paniculata at the same time.关键词
液相色谱-串联质谱法/穿心莲/农残Key words
LC-MS/MS/Andrographis paniculata/pesticide residue分类
医药卫生引用本文复制引用
梁佳,刘芳芳,陈文北,钟仁清,王丹,刘地发,张寿文..LC-MS/MS法同时测定穿心莲中6种农药残留量[J].特产研究,2024,46(2):128-133,140,7.基金项目
江西省主要学科学术和技术带头人培养计划-领军人才项目(20225BCJ22018) （20225BCJ22018）
省级中小企业发展专项-中药注射剂全产业链安全性控制关键共性技术的研究与推广 （）
