塔里木大学学报2024，Vol.36Issue(1)：22-27,6.DOI:10.3969/j.issn.1009-0568.2024.01.003
连翘酯苷A负调控HMGB1的表达抑制bEECs炎性损伤作用研究
Forsythoside A inhibits the inflammatory damage effect of bEECs by negatively regulating the expression of HMGB1
摘要
Abstract
Endometritis in dairy cows is one of the major diseases causing huge economic losses in the dairy industry.Recent studies have shown that forsythoside A,extracted from the traditional Chinese herb forsythia,has a wide range of biological activities.The aim of this study was to elucidate the inhibitory effect of forsythoside A on Escherichia coli endotoxin mediated inflammatory damage in bo-vine endometrial epithelial cells(bEECs)as well as the possible molecular mechanisms in vitro.The results showed that conotoxin sig-nificantly attenuated the damage effect of bEECs in a model of LPS-induced inflammatory injury in bEECs.Results of qPCR showed that forsythoside A significantly inhibited the release of pro-inflammatory cytokines(TNF-α,IL-6 and IL-1β)in a dose-dependent manner.Results from immunofluorescence and immunoblotting confirmed that coniferin significantly inhibited the expression of high mobility protein(HMGB1),which in turn blocked the activation of nuclear factor NF-κB.In conclusion,the findings suggest that forsythoside A protects against LPS-induced inflammatory injury in bEECs by negatively regulating HMGB1 expression,mediated by the NF-κB pathway.关键词
连翘酯苷A/子宫内膜上皮细胞/高迁移率蛋白1/炎性损伤Key words
forsythoside A/bEECs/HMGB1/inflammatory damage分类
医药卫生引用本文复制引用
苏朵朵,马蓓,丁泽,周海青,张佳妮,山丽娜,刘俊峰..连翘酯苷A负调控HMGB1的表达抑制bEECs炎性损伤作用研究[J].塔里木大学学报,2024,36(1):22-27,6.基金项目
新疆生产建设兵团指导性科技计划项目(TDZKCX202209/2022ZD100) （TDZKCX202209/2022ZD100）
塔里木大学大学生创新创业训练计划项目(202210757001) （202210757001）
