萱草愈伤组织分化的研究OA

In order to solve the problem that Hemerocallis fulva healing tissues only grew and did not differentiate into buds,and to improve the reproduction coefficient,the healing tissues of selected JH2-2 hemerocallis successional culture were inoculated and cul-tured by using different ratios of exogenous hormones of 6-BA and IBA,and the healing tissues with different colours of healing tis-sues were made into paraffin slices for observation.The experimental results showed that on the 6th day of inoculation and culture,small green buds began to differentiate from the injured tissue one after another.On the twenty-first day,the differentiation rate of callus reached the maximum one after another.At the same time,some callus appeared vitrification,and there was no sign of differen-tiation and germination in the follow-up.The exogenous hormone 6-BA had a greater effect on the differentiation of hemerocallis than IBA.The proportion of medium suitable for differentiation culture of hemerocallis was MS+sucrose 30 g·L-1+agar 6 g·L-1+6-BA 1.0 mg·L-1+IBA 0.2 mg·L-1,pH Value was 6.5,and the differentiation rate reached 100%under this condition.It was found that one of them was embryogenic callus,which was spherical and granular in shape,the cells were arranged neatly and closely,and the nuclei were stained deeply.The other was non embryogenic callus,with soft and rough appearance,no fixed shape,uneven cell size and no fixed shape,and unclear nucleus.In the process of hemerocallis tissue culture,priority could be given to the inoculation of milky-white and green active healing tissues,which was of great significance for improving the differentiation and reproduction rates of hemerocallis.The results of the study can provide certain technical support for optimizing the technical system of hemerocallis in vitro fast propagation and further creating new varieties.