陆地棉TRM基因家族的鉴定及纤维品质相关优异单倍型分析OA北大核心CSTPCD

[Objective]Identification of TRM gene family and fiber quality related excellent haplotype a-nalysis in Gossypium hirsutum L.[Methods]Bioinformatics was used to analyze the evolutionary relationship,physicochemical properties and cluster expression of TRM gene families.Candidate genes regulating fiber quali-ty traits(fiber length,strength,and Macron value)were screened by gene haplotype analysis.[Results]The a-nalysis of physicochemical properties showed that the amino acids encoded by this family gene ranged from 376-1,093,with isoelectric points ranging from 4.64-9.56.Subcellular localization predicted that up to 60 TRM family genes were localized in the nucleus.The results of cis-acting element analysis showed that TRM gene family contained more elements related to light response,hormone response,stress response and growth and de-velopment.Transcriptome data showed that 60 TRM family genes were expressed preferentially during fiber de-velopment,which might regulate cotton fiber development.Furthermore,1,245 upland cotton materials were used to genotype TRM,and the number of haplotypes of each gene was found to be 1-8,and 14,18 and 15 ex-cellent haplotype TRM genes related to fiber length,strength and Macron value were screened,respective-ly.Among them,11 genes had excellent haplotypes of length,strength and micronotype.The Hap_4 haplotype of GH_D09G0775 and the Hap_3 haplotype of GH_D03G1434 increased fiber length and strength while decrea-sing the Macron value.[Conclusion]In this study,75 TRM family members were identified on 24 chromo-somes in upland cotton(Gossypium hirsutum L.),which were systematically divided into cluster evolutionⅠ-Ⅲ3 subtribe.