新疆农业科学2024，Vol.61Issue(3)：521-536,16.DOI:10.6048/j.issn.1001-4330.2024.03.001
陆地棉TRM基因家族的鉴定及纤维品质相关优异单倍型分析
Identification of TRM gene family and fiber quality related excellent haplotype analysis in Gossypium hirsutum L.
摘要
Abstract
[Objective]Identification of TRM gene family and fiber quality related excellent haplotype a-nalysis in Gossypium hirsutum L.[Methods]Bioinformatics was used to analyze the evolutionary relationship,physicochemical properties and cluster expression of TRM gene families.Candidate genes regulating fiber quali-ty traits(fiber length,strength,and Macron value)were screened by gene haplotype analysis.[Results]The a-nalysis of physicochemical properties showed that the amino acids encoded by this family gene ranged from 376-1,093,with isoelectric points ranging from 4.64-9.56.Subcellular localization predicted that up to 60 TRM family genes were localized in the nucleus.The results of cis-acting element analysis showed that TRM gene family contained more elements related to light response,hormone response,stress response and growth and de-velopment.Transcriptome data showed that 60 TRM family genes were expressed preferentially during fiber de-velopment,which might regulate cotton fiber development.Furthermore,1,245 upland cotton materials were used to genotype TRM,and the number of haplotypes of each gene was found to be 1-8,and 14,18 and 15 ex-cellent haplotype TRM genes related to fiber length,strength and Macron value were screened,respective-ly.Among them,11 genes had excellent haplotypes of length,strength and micronotype.The Hap_4 haplotype of GH_D09G0775 and the Hap_3 haplotype of GH_D03G1434 increased fiber length and strength while decrea-sing the Macron value.[Conclusion]In this study,75 TRM family members were identified on 24 chromo-somes in upland cotton(Gossypium hirsutum L.),which were systematically divided into cluster evolutionⅠ-Ⅲ3 subtribe.关键词
陆地棉/基因家族/基因表达/纤维品质/单倍型Key words
Gossypium hirsutum L./gene family/gene expression/fiber quality/haploidgenotype分类
农业科技引用本文复制引用
王凯迪,陈全家,高晨旭,裴文锋,杨书贤,张文庆,宋吉坤,马建江,王莉,于霁雯..陆地棉TRM基因家族的鉴定及纤维品质相关优异单倍型分析[J].新疆农业科学,2024,61(3):521-536,16.基金项目
河南省农业良种联合攻关项目(2022010301) （2022010301）
新疆维吾尔自治区自然科学基金项目(2021D01B113) （2021D01B113）
中央级公益性科研院所基本科研业务费专项(1610162022013) Henan Province Agricultural Improved Seed Joint Research Project(2022010301) （1610162022013）
Natural Science Foundation of Xinjiang Uygur Autonomous Region(2021D01B113) （2021D01B113）
Basic Research Funds for Public Welfare Research Institutes of the Central Government(1610162022013) （1610162022013）
