现代妇产科进展2024，Vol.33Issue(4)：282-286,5.DOI:10.13283/j.cnki.xdfckjz.2024.04.003
UPLC-MS/MS法检测血浆内源性大麻素在预测先兆流产孕妇妊娠结局中的应用
Application of UPLC-MS/MS method in detecting endogenous cannabinoids in plasma for predicting pregnancy outcomes in women with threatened miscarriage
摘要
Abstract
Objective:To study the periodic variation of endogenous cannabinoids(ECs)in peripheral blood of pregnant women in China,and to analyze the clinical application value of predicting the pregnancy outcome of pregnant women with threatened abortion based on the level of endogenous cannabinoids in early pregnancy.Methods:A total of 1080 pregnant women who underwent prenatal examination in the First Affiliated Hospital of Shandong First Medical University from May 2020 to May 2022 were selected as the research objects,including 880 pregnant women with threatened abortion in early pregnancy and 200 normal pregnant women.The level of endogenous cannabinoids in blood was detected by ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Logistic regression was used to analyze the risk factors of threatened abortion developing into inevitable abortion,and ROC curve was used to evaluate the performance of progesterone,β-HCG and AEA in predicting the pregnancy outcome of pregnant women with threatened abortion.Results:The average level of AEA in peripheral blood of pregnant women with inevitable abortion was(3.96±2.04)nmol/L,which was significantly higher than that of pregnant women with continuous pregnancy[(2.16±2.00)nmol/L](P<0.001).Age,AEA,progesterone,β-HCG,gestational age and vaginal bleeding were risk factors for the development of threatened abortion into inevitable abortion(P<0.05).When the threshold was 2.565nmol/L,the accuracy of endogenous cannabinoid AEA in early pregnancy for predicting the pregnancy outcome of pregnant women with threatened a-bortion was 86.7%,which had the best prediction performance.Conclusion:The determination of endogenous cannabinoid AEA level in peripheral blood of pregnant women is an effective tool to predict the pregnancy outcome of patients with threatened abortion.关键词
超高效液相色谱串联质谱(UPLC-MS/MS)/先兆流产/难免流产/内源性大麻素Key words
Ultra performance liquid chromatography tandem mass spectrometry(UP-LC-MS/MS)/Threatened abortion/Inevitable abortion/Endogenous cannabinoids分类
医药卫生引用本文复制引用
王玉秀,刘立东,郭伟..UPLC-MS/MS法检测血浆内源性大麻素在预测先兆流产孕妇妊娠结局中的应用[J].现代妇产科进展,2024,33(4):282-286,5.基金项目
山东省自然科学基金青年项目(No:ZR2020QH246) （No:ZR2020QH246）
