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响应面优化超声提取绿色棉纤维总黄酮OA北大核心CSTPCD

Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization

中文摘要英文摘要

[目的]优化超声提取绿色棉纤维中总黄酮工艺条件,为合理利用及鉴别天然绿色棉纤维提供依据.[方法]以天然绿色棉纤维为原料,利用超声空化效应加速黄酮溶解于乙醇中,在单因素试验基础上,结合Box-Benhnken中心组合原理及响应面设计优化试验条件.[结果]料液比为 1∶50 g/mL时,总黄酮得率达到最大值,提取最适料液比为1∶50 g/mL,响应面试验中建立的模型的CV值=0.87%,影响绿色棉纤维总黄酮得率的顺序为提取时间>乙醇浓度>料液比,A料液比为1∶50 g/mL、B乙醇浓度50%、C提取时间50 min为最佳提取条件.[结论]超声波法提取绿色棉纤维总黄酮的最佳条件参数为料液比1∶50 g/mL、乙醇浓度50%、提取时间50 min;获得平均总黄酮得率为5.25%.

[Objective]This study is to optimize ultrasonic extraction conditions of total flavonoids in NGC fibers,in the hope of providing the basis for reasonable utilization and identification of NGC fibers.[Methods]The NGC fibers were used as raw materials and were utilized for total flavonoids extract by ultra-sonic cavitation effect to accelerate the dissolution of flavonoids in ethanol,to optimize the experimental condi-tions with Box-Benhnken center combination principle and response surface design.[Results]The optimal extraction conditions were as follows:solid-liquid ratio of 1∶50 g/mL,ethanol concentration of 50%,and ex-traction time of 50 min.Under these conditions,the average yield of total flavonoids was 5.25%,which was the best condition for the extraction of total flavonoids from NGC fibers by ultrasonic method.[Conclusion]The optimal condition Parameter for ultrasonic extraction of total flavonoids from green cotton fibers is a liquid to material ratio of 1∶50 g/mL,ethanol concentration50%,extraction time50 min,obtaining an average total fla-vonoid yield of 5.25%.

陈沛;鲍军秀;王斐;汤寿伍;刘海峰;李鸿彬

石河子大学生命科学学院,新疆石河子 832003石河子大学生命科学学院,新疆石河子 832003||绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子 832000||新疆植物药资源利用教育部重点实验室,新疆石河子 832000中国彩棉(集团)股份有限公司,乌鲁木齐 830001石河子大学生命科学学院,新疆石河子 832003||绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子 832000||新疆植物药资源利用教育部重点实验室,新疆石河子 832000||中国彩棉(集团)股份有限公司,乌鲁木齐 830001

农业科学

绿色棉纤维总黄酮响应面法最佳提取条件超声波细胞破碎

green cotton fibertotal flavonoidsresponse surface methodoptimum extraction conditionultrasionic cell-break

《新疆农业科学》 2024 (003)

576-583 / 8

新疆维吾尔自治区创新环境建设专项(2020D14035);新疆生产建设兵团科技攻关与成果转化项目(2016AC017) Innovation Environment Construction Project of Xinjiang Uygur Autonomous Region(2020D14035);Tackling Hard-Nut Problems in Science and Technology and Research Results Transformation Project of XPCC(2016AC017)

10.6048/j.issn.1001-4330.2024.03.006

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