西北林学院学报2024，Vol.39Issue(2)：21-27,7.DOI:10.3969/j.issn.1001-7461.2024.02.03
干旱胁迫下沙棘CMO基因的时空表达模式与蛋白结构预测
Spatiotemporal Expression Patterns and Protein Structure Prediction of Seabuckthorn CMO Gene Under Drought Stress
摘要
Abstract
CMO gene is a key gene for the synthesis of betaine in Hippophae rhamnoides.It plays an im-portant role in drought resistance of plants.Taken the roots,stems and leaves of wild H.rhamnoides sub-sp.sinensis Rousi occurring in Qinghai as the experimental materials,the CMO gene and its full-length se-quence were cloned and bioinformatics was analyzed,the spatiotemporal expression patterns of CMO genes in different tissue parts of sea buckthorn plant under different degrees of drought stress were studied.The results were reported as follows.The total length of CMO gene is 1 566 bp,the ORF length is 1 364 bp,en-coding 454 amino acids,the molecular weight is 51.41 ku,the isoelectric point is 6.78;CMO protein sub-cells are localized in chloroplasts,no signal peptides,and the protein secondary structure is mainly irregular curling;CMO protein has a number of various types of functional sites,which provide a guarantee for the realization of protein function;in addition,CMO protein has strong hydrophilicity and no transmembrane helix region;the three-dimensional structure of CMO protein consists of α helix,β sheet,and random coil.The spatiotemporal expression pattern study showed that the expression of CMO gene was obviously af-fected by drought stress,and the expression of CMO gene showed a gradual increase with the extension of stress time,and did not decrease until the end of stress or after rehydration.The expression of CMO gene in different tissue parts was different,and the expression size was in the order of root＞leaf＞stem.This study analyzes the CMO gene of sea buckthorn in order to provide reference significance for improving the drought resistance of H.rhamnoides and the in-depth study of plant CMO gene.关键词
沙棘/干旱胁迫/CMO基因/蛋白质结构Key words
Hippophae rhamnoides subsp.sinensis/drought stress/CMO gene/protein structure分类
农业科技引用本文复制引用
刘青青,马玉花,董佳伟,冶贵生,张丹,杨开宇..干旱胁迫下沙棘CMO基因的时空表达模式与蛋白结构预测[J].西北林学院学报,2024,39(2):21-27,7.基金项目
国家自然科学基金(31660071) （31660071）
青海省科技厅项目(2017-ZJ-734) （2017-ZJ-734）
青海省"高端创新人才千人计划"项目. （）
