珙桐体细胞胚胎发生技术研究OA北大核心CSTPCD

The effects of basic medium types on the callus induction of Davidia involucrata and plant growth regulators on embryogenic callus induction and somatic embryo development were studied.A cul-ture scheme for the regeneration of D.involucrata plants by somatic embryogenesis with immature zygotic embryos as explants was preliminarily established.The results showed that callus could be induced on MS,1/2MS and WPM basic media containing 3％sucrose,0.3％gel,800 mg/L hydrolyzed casein,400 mg/L L-glutamine,and MS+2.0 mg/L 2,4-D+0.5 mg/L 6-BA had the highest induction rate of 94.0％.The em-bryogenic callus was induced on MS+0.5 mg/L NAA+1.0 mg/L 6-BA medium.On MS+0.5 mg/L NAA+1.0 mg/L 6-BA medium,the induction rate of somatic embryos reached 22.9％.Mature somatic embryos could germinate normally on the germination medium of 1/2MS+0.5 mg/L 6-BA+0.25 mg/L IBA,and then transformed into complete plantlets on the germination medium supplemented with 1 g/L activated carbon.Histocytological observation showed that the somatic embryos originated from embryo-genic callus and underwent the development stages of globular-,heart-,torpedo-and cotyledonary-stage,which was similar to the formation process of zygotic embryos under natural conditions.