西北林学院学报2024，Vol.39Issue(2)：70-78,9.DOI:10.3969/j.issn.1001-7461.2024.02.09
珙桐体细胞胚胎发生技术研究
Somatic Embryogenesis in Davidia involucrata
摘要
Abstract
The effects of basic medium types on the callus induction of Davidia involucrata and plant growth regulators on embryogenic callus induction and somatic embryo development were studied.A cul-ture scheme for the regeneration of D.involucrata plants by somatic embryogenesis with immature zygotic embryos as explants was preliminarily established.The results showed that callus could be induced on MS,1/2MS and WPM basic media containing 3％sucrose,0.3％gel,800 mg/L hydrolyzed casein,400 mg/L L-glutamine,and MS+2.0 mg/L 2,4-D+0.5 mg/L 6-BA had the highest induction rate of 94.0％.The em-bryogenic callus was induced on MS+0.5 mg/L NAA+1.0 mg/L 6-BA medium.On MS+0.5 mg/L NAA+1.0 mg/L 6-BA medium,the induction rate of somatic embryos reached 22.9％.Mature somatic embryos could germinate normally on the germination medium of 1/2MS+0.5 mg/L 6-BA+0.25 mg/L IBA,and then transformed into complete plantlets on the germination medium supplemented with 1 g/L activated carbon.Histocytological observation showed that the somatic embryos originated from embryo-genic callus and underwent the development stages of globular-,heart-,torpedo-and cotyledonary-stage,which was similar to the formation process of zygotic embryos under natural conditions.关键词
珙桐/胚性愈伤组织/体细胞胚/植株再生/组织细胞学Key words
Davidia involucrata/embryogenic callus/somatic embryos/plantlet regeneration/histocytology分类
农业科技引用本文复制引用
令狐高曼,季晓莲,段榕怡,刘睿,戴钊,董嘉明,徐金颢,康永祥..珙桐体细胞胚胎发生技术研究[J].西北林学院学报,2024,39(2):70-78,9.基金项目
秦岭珍稀植物红豆杉、珙桐高效繁育技术研究与示范(SXLK2021-01-03). （SXLK2021-01-03）
