中国北方青杨派杨树遗传多样性研究OA北大核心CSTPCD

Tacamahaca is a type of ecological and timber tree species widely distributed in China.At pres-ent,little studies have been carried out on the effective resource assessment,utilization,and protection.Taking 112 samples obtained from 19 Tacamahaca populations in Northern China as research materials,this study assessed the genetic diversity and genetic structure by using 15 microsatellite markers.The re-sults showed that total number of alleles(Na)was 118,average number of effective allele(Ne)was 3.439,observed heterozygosity(Ho)and expected heterozygosity(He)was 0.392 and 0.347,respective-ly,and information index(I)was 0.582,indicating that a high level of genetic diversity among 19 Tacama-haca populations.The genetic differentiation coefficient(Fst)was 0.39,suggesting that there is significant differentiation among 19 Tacamahaca populations.Niteen Tacamahaca populations were divided into the two clusters by structure and cluster analysis.There was a similar genetic relationships between P.ussu-riensis population and P.koreana population,as well as P.gansuensis population and P.simonii popula-tion.Nine populations of P.cathayana can be divided into the three clusters,the genetic distance was sig-nificantly correlated with geographical distance and climatic distance,respectively(r=0.758,P=0.010;r=0.330,P=0.04),indicating that the genetic differentiation of P.cathayana populations is influenced by geographical isolation and climatic differences.This study provides a theoretical basis for the genetic im-provement,germplasm conservation and utilization of Tacamahaca resources,and provides an important reference for the study of phylogenetic and evolutionary mechanism of Tacamahaca.