西北林学院学报2024，Vol.39Issue(2)：97-105,9.DOI:10.3969/j.issn.1001-7461.2024.02.13
西双版纳热带森林恢复对土壤反硝化N2O排放的影响
Influence of Tropical Forest Restoration on Spatiotemporal Variations in N2O Emissions from Soil Denitrification
摘要
Abstract
This study aimed to explore the effect of tropical forest restoration on the spatiotemporal dynam-ics of N2O emissions from soil denitrification.The continuous indoor-culture method(pure-oxygen inhibi-tion denitrification)and high-throughput metagenomic sequencing technology were used to determine the N2O emissions and microbial diversity in tropical forests(i.e.,Mallotus paniculatus,Mellettia leptobota,and Syzygium oblatum communities)at different restoration stages in Xishuangbanna.We also determined the linkage of denitrifying N2O emission with soil microbial diversity and chemical properties.The results showed that 1)tropical forest restoration significantly affected the N2O emissions from soil denitrification(P＜0.05).The average emission rates were ranked as S.oblatum(303.16±37.57)＞M.leptobota(251.38±28.37)＞M.paniculatus(193.45±26.76 μg·kg-1·h-1).2)N2O emission rates of denitrifica-tion had a significant spatio-temporal variation.They were significantly higher in the wet season(June and September)than in the dry season(March and December);the range of the fluctuations in N2 O emission rates from wet to dry season ranked as M.paniculatus(2.94 times)＞S.oblatum(2.50 times)＞M.leptob-ota(2.22 times);the vertical variation decreased along the soil profile.3)The explanatory order of soil wa-ter content,microbial biomass carbon and Shannon diversity for the changes of denitrifying N2O emissions at the three restoration stages was ranked as S.oblatum(68.77％,70.45％,and 97.87％)＞M.leptobota(62.54％,57.16％,and 96.8％)＞M.paniculatus(54.56％,46.46％,and 93.17％).4)The result of prin-cipal component analysis showed that total organic carbon,readily oxidizable carbon,Shannon index,water content,and microbial carbon were the main factors regulating the N2O emissions from denitrification.The contribution of total,nitrate,ammonium,and hydrolyzed nitrogen,Chao index,and Simpson index ranked the second.Tropical forest restoration can regulate the spatiotemporal variations in N2O emission during soil denitrification,probably through mediating denitrifying bacterial diversity,water content,and carbon pool components.关键词
热带森林/次生演替/反硝化作用/反硝化微生物/N2O排放Key words
tropical forest/secondary succession/denitrification/denitrifying microorganism/N2O emission分类
农业科技引用本文复制引用
张路路,罗双,李瑞,夏佳慧,杨胜秋,兰梦杰,王邵军,张昆凤,樊宇翔,解玲玲,肖博,王郑钧,郭志鹏,郭晓飞..西双版纳热带森林恢复对土壤反硝化N2O排放的影响[J].西北林学院学报,2024,39(2):97-105,9.基金项目
国家自然科学基金项目(32060281,31660191) （32060281,31660191）
云南省一流学科建设项目(云转发[2022]73号) （云转发[2022]73号）
云南省教育厅科学研究基金项目(2019Y0136). （2019Y0136）
