信阳师范学院学报（自然科学版）2024，Vol.37Issue(2)：222-227,6.DOI:10.3969/j.issn.1003-0972.2024.02.014
银钟花根尖染色体制片及核型分析
Optimization of Chromosome Preparation Technology and Karyotype Analysis of Perkinsiodendron macgregorii(Chun)P.W.Fritsch
摘要
Abstract
The root tips of P.macgregorii seeds was used as the research material to investigate the effects of different pretreatment reagents,pretreatment time and dissociation methods on the chromosome preparation of P.macgregorii root tips.A set of chromosome preparation technique of P.macgregorii was obtained and used as the basis for karyotype analysis to reveal its karyotype characteristics,providing a foundation for the conservation and genetic research of P.macgregorii.The results showed that pretreatment with 0.003 mol/L 8-hydroxyquinoline in the dark for 6 h,fixation with Carnoy's fixative for 10 h,dissociation with 1 mol/L HCl in a constant temperature water bath at 60℃for 12 min,and staining with 20%carbol fuchsin could obtain high-quality chromosome squash of P.macgregorii with good dispersion and clear morphology.The chromosome number of P.macgregorii was 2n=24,and the karyotype formula was 2n=24=20m+4Sm,with an asymmetry coefficient of 55.77%.The karyotype was classified as type 1B.关键词
银钟花/染色体/制片技术/核型分析/根尖Key words
Perkinsiodendron macgregorii(Chun)P.W.Fritsch/chromosome/filming technique/karyotype analysis/root tip分类
生物科学引用本文复制引用
闫明慧,孙文光,张银,宁汪洋,刘彬文..银钟花根尖染色体制片及核型分析[J].信阳师范学院学报（自然科学版）,2024,37(2):222-227,6.基金项目
国家自然科学基金项目(31800276) （31800276）
河南省高等学校重点科研项目(19A180029) （19A180029）
信阳师范学院"南湖学者奖励计划"青年项目 （）
