云南师范大学学报（自然科学版）2024，Vol.44Issue(2)：54-60,7.DOI:10.7699/j.ynnu.ns-2024-025
快速滤过型净化结合超高效液相色谱-串联质谱法测定茶叶中5种农药残留量
Determination of 5 Pesticide Residues in Tea by Multi-plug Filtration Purification Cleanup Method Combined with Ultra Performance Liquid Chromatography-tandem Mass Spectrometry
杜鑫 1易珊珊 1何家国 1覃明丽 1苏旭 1陈绍辉 1蒋金芳1
作者信息
- 1. 南充农产品质量监测检验中心,四川南充 637000
- 折叠
摘要
Abstract
A method was established for simultaneous determination of thiamethoxam,fenpyroximate,propargite,buprofezin and abamectin residues in tea,which was based on multi-plug filtration cleanup(m-PFC)column combined with Ultra Performance Liquid Chromatography-tandem Mass Spectrome-try(UPLC-MS/MS).The samples were firstly extracted with acetic acid acetonitrile after being fully wetted with water.QuEChERS salt packs were added to the system to separate into organic and aque-ous phases,then the upper fraction was purified on a m-PFC column.The target drug residues were lastly quantified by the matrix-matched external standard method using UPLC-MS/MS in the multiple reaction monitoring(MRM)mode with positive electrospray ionization(ESI+).The results showed 5 pesticide residues had good linearity in the range of 1.00～100.00 μg·L-1 with correlation coefficient(r)above 0.999.The detection limits were in the range of 1.2～2.2 μg·kg-1,and the quantitation lim-its were 5.0 μg·kg-1.At the addition level of 5.00～250 μg·kg-1,the recoveries ranged from 71.5％to 109.9％,with relative standard deviations(RSDs)of 2.6％～8.4％(n=3).关键词
快速滤过型净化/农药残留/超高效液相色谱-串联质谱/茶叶Key words
Multi-plug filtration cleanup(m-PFC)/Pesticide residues/UPLC-MS/MS/Tea分类
化学化工引用本文复制引用
杜鑫,易珊珊,何家国,覃明丽,苏旭,陈绍辉,蒋金芳..快速滤过型净化结合超高效液相色谱-串联质谱法测定茶叶中5种农药残留量[J].云南师范大学学报（自然科学版）,2024,44(2):54-60,7.
