亚热带植物科学2024，Vol.53Issue(1)：12-21,10.DOI:10.3969/j.issn.1009-7791.2024.01.002
血叶兰幼苗对干旱胁迫的生理响应及其抗旱性指标筛选
Physiological Response of Ludisia discolor Seedlings to Drought Stress and Screening of Drought Resistance Indicators
摘要
Abstract
In order to investigate the tolerance of Ludisia discolor to drought stress,the experiments potted water control to simulate drought were used to study the changes of growth,physiological and biochemical indicators,and bioactive components in L.discolor seedlings with around 6 months of age under different water treatments.The main response factors to drought resistance were evaluated through correlation analysis(CA)and principal component analysis(PA),which would provide some references for the water management under conservation and cultivation of L.discolor.The results showed that with the increase of drought severity,the height of L.discolor seedlings showed a downward trend compared to the control group,but there was no significant effect on stem thickness.The relative water content of leaves decreased,and the content of chloroplast pigment(Chl)first increased and then decreased,while the content of anthocyanin(ACN)showed an upward trend.Under drought stress,the activities of peroxidase(POD)and superoxide dismutase(SOD)showed a trend of first increasing and then decreasing.The contents of malondialdehyde(MDA),soluble sugar(SS),and the bioactive substances such as total flavonoids(TF),total phenols(TP),and polysaccharides(PS)all increased.Through correlation analysis and principal component analysis,it was found that MDA,SS,PS,and relative water content could be used as the preferred evaluation indicators,and in addition,stem thickness and SOD activity could be used as auxiliary indicators for drought resistance evaluation in L.discolor.In summary,the results indicated that moderate drought stress(soil moisture content 50%-55%)could promote the synthesis and accumulation of osmotic regulating substances and bioactive components of L.discolor,At the same time,to improve the quality of L.discolor,it was recommended to carry out short-term severe drought(soil moisture content 15%-20%)before harvest so that the biological activities of L.discolor could be enhanced.These findings had practical guiding significance for cultivation management.关键词
血叶兰/干旱胁迫/生理特性/生物活性成分Key words
Ludisia discolor/drought stress/physiological characteristics/biologically active ingredients分类
农业科技引用本文复制引用
许丽丽,孟新亚,尤燕平,宋希强,陈耀丽,钟云芳..血叶兰幼苗对干旱胁迫的生理响应及其抗旱性指标筛选[J].亚热带植物科学,2024,53(1):12-21,10.基金项目
海南省自然科学基金项目(322MS019) （322MS019）
