现代畜牧科技Issue(4)：42-45,4.DOI:10.19369/j.cnki.2095-9737.2024.04.011
1株5型副猪格拉菌的分离鉴定及生物学特性研究
Isolation,Identification and Biological Characteristics of a Strain 5 of Glaesserella parasuis
摘要
Abstract
Glaesserella parasuis is an important pathogen in pigs,posing a significant threat to the global swine industry and causing substantial economic losses.Conducting isolation,identification,and characteristic analysis of GPS can help provide a basis for clinical diagnosis,treatment,and prevention.In this experiment,the bacteria were isolated,cultured and identified from clinical piglet disease feed,and the isolated strains were identified and analyzed by Gram staining,biochemical identification,PCR amplification and sequencing comparison,genetic evolution analysis,genotyping,and drug susceptibility test and pathogenicity study were carried out.The results showed that Glaesserella parasuis was isolated and its homology was 92.3%～95.6%by genetic evolution analysis.The genotype of the isolates was serotype 5.The isolates were sensitive to doxycycline,amoxicillin.Resistant to neomycin,tylosin,cotrimoxazole,cefalothiophene.The pathogenicity test in mice showed that it was pathogenic.The relevant results of this experiment provide theoretical basis for clinical prevention and treatment of Glaesserella parasuis.关键词
副猪格拉菌/分离/鉴定/药敏试验/致病性Key words
Glaesserella parasuis/isolation/identification/drug susceptibility test/pathogenicity分类
农业科技引用本文复制引用
田野,时永强,张飞,杜鹏飞,陈玲,蒋颖..1株5型副猪格拉菌的分离鉴定及生物学特性研究[J].现代畜牧科技,2024,(4):42-45,4.基金项目
国家重点研发计划:国家生物安全实物核心资源库支撑关键技术研究(2022YFC2602202) （2022YFC2602202）
