现代畜牧科技Issue(4)：53-55,3.DOI:10.19369/j.cnki.2095-9737.2024.04.014
不同酶消化法提取猪原代肝细胞的效果比较
Comparison of the Effect of Different Enzyme Digestion Methods on Extraction of Porcine Primary Hepatocytes
摘要
Abstract
Porcine hepatocytes are one of the target cells of porcine circovirus.An efficient method to simply and quickly isolate hepatocytes is vital to investigate pathogenesis of porcine circovirus disease.In this article,the effects of trypsin,type II collagenase,type IV collagenase,type II collagenase and type IV collagenase mixture on extraction of porcine primary hepatocytes were compared by enzyme digestion in vitro.Results show that collagenase mixture group exhibited the most efficient cell digestion,whereas trypsin group did the opposite.Hepatocytes isolated from type II and type IV collagenase digestion represented neatly arranged shapes and clear boundaries.关键词
猪/原代肝细胞/提取/消化/圆环病毒/圆环病毒病Key words
piglet/primary hepatocytes/extract/digestion/circovirus/circovirus disease分类
农业科技引用本文复制引用
周徐倩,董磊..不同酶消化法提取猪原代肝细胞的效果比较[J].现代畜牧科技,2024,(4):53-55,3.基金项目
国家自然科学基金项目:复杂器官与组织在脾脏内的功能性再生(32230056) （32230056）
