针刺研究2024，Vol.49Issue(4)：376-383,8.DOI:10.13702/j.1000-0607.20230578
艾灸对ApoE-/-动脉粥样硬化小鼠SIRT1/FOXO3a信号通路的影响
Effect of moxibustion on the SIRT1/FOXO3a signaling pathway in ApoE-/-mice with atherosclerosis
摘要
Abstract
Objective To observe the effects of moxibustion on blood lipid metabolism,pathological morphology of thoracic aorta,and the expression of silent information regulator 1(SIRT1)and forkhead box transcription factor O3a(FOXO3a)in ApoE-/-atherosclerosis(AS)mice,so as to explore the potential mechanism of moxibustion in preventing and treating AS.Methods Ten C57BL/6J mice were fed a normal diet as the control group,and 30 ApoE-/-mice were fed a high-fat diet to establish the AS model,which were randomly divided into the model group,simvastatin group,and moxibustion group,with 10 mice in each group.From the first day of modeling,mice in the moxibustion group received mild moxibustion treatment at"Shenque"(CV8),"Yinlingquan"(SP9),bilateral"Neiguan"(PC6)and"Xuehai"(SP10)for 30 min per time;the mice in the simvastatin group were given simvastatin orally(2.5 mg·kg-1·d-1),with both treatments given once daily,5 times a week,with a total intervention period of 12 weeks.The body weight and general condition of the mice were observed and recorded during the intervention period.After the intervention,the contents of serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were measured using an automated biochemistry analyzer.Hematoxylin eosin(HE)staining was used to observe the pathological morphology of the thoracic aorta.ELISA was used to measure the contents of serum oxidized low-density lipoprotein(ox-LDL)and superoxide dismutase(SOD)activity.Western blot and real-time fluorescent quantitative PCR analysis were used to detect the expression levels of SIRT1 and FOXO3a protein and mRNA in the thoracic aorta.Results Compared with the control group,body weight at the 8th and 12th week,serum TC,TG,LDL-C,and ox-LDL contents of the model group mice were significantly increased(P<0.05,P<0.01),while the HDL-C contents,SOD activity,and the expression levels of SIRT1 protein and mRNA in the thoracic aorta were significantly decreased(P<0.05,P<0.01).HE staining showed thickening of the aortic intima,endothelial cell degeneration,swelling,and shedding.Compared with the model group,body weight at the 8th and 12th week,serum TC,TG,LDL-C,and ox-LDL contents of mice in the simvastatin group and moxibustion group were significantly decreased(P<0.01),while the serum SOD activity,expression levels of SIRT1 protein and mRNA in the thoracic aorta were significantly increased(P<0.01).The HDL-C contents were significantly increased in the simvastatin group(P<0.05).The thoracic aortic structure was more intact in both groups,with a more regular lumen and orderly arrangement of the elastic membrane in the media,and a slight amount of endothelial cell degeneration and swelling in the intima.There was no significant difference in the evaluated indexes between the moxibustion group and the simvastatin group and the pathological changes in the thoracic aorta were similar between the two groups.Conclusion Moxibustion can reduce the body weight of AS model mice,regulate lipid levels,repair vascular intima,and alleviate endothelial damage.Its mechanism of action may be related to the regulation of the SIRT1/FOXO3a signaling pathway to improve oxidative damage.关键词
动脉粥样硬化/温和灸/沉默信息调节因子1/叉头框转录因子O3aKey words
Atherosclerosis/Mild moxibustion/Silent information regulator 1/Forkhead box transcription factor O3a引用本文复制引用
闫朝勃,伍先明,张宁,杨志虹,朱洲,喻华梅,贾晓琪,杨孝芳..艾灸对ApoE-/-动脉粥样硬化小鼠SIRT1/FOXO3a信号通路的影响[J].针刺研究,2024,49(4):376-383,8.基金项目
国家自然科学基金项目(No.82160941、82360978) （No.82160941、82360978）
贵州省科技计划项目(No.黔科合基础-ZK[2022]一般499) （No.黔科合基础-ZK[2022]一般499）
贵州省卫生健康委科学技术基金项目(No.gzwkj2023-019) （No.gzwkj2023-019）
贵州省中医药管理局项目(No.QZYY-2021-021) （No.QZYY-2021-021）
