针刺研究2024，Vol.49Issue(4)：384-390,7.DOI:10.13702/j.1000-0607.20230024
电针对帕金森病大鼠中脑黑质Sirt3/NLRP3/GSDMD信号通路的影响
Effects of electroacupuncture on Sirt3/NLRP3/GSDMD signaling pathway in the substantia nigra of midbrain of rats with Parkinson's disease
摘要
Abstract
Objective To observe the effects on tyrosine hydroxylase(TH),α-synaptic nucleoprotein(α-syn),sirtuin 3(Sirt3),NOD-like receptor 3(NLRP3)and gasdermin-D(GSDMD)in the substantia nigra of midbrain after electroacupuncture(EA)at"Fengfu"(GV16),"Taichong"(LR3)and"Zusanli"(ST36)in rats of Parkinson's disease(PD),so as to explore the mechanism of EA in treatment of PD.Methods SD rats were randomly divided into control,model and EA groups,with 10 rats in each group.The PD model was established by injecting rotenone into the neck and back,lasting 28 days.In the EA group,EA was applied to GV16,LR3 and ST36,30 min each time,once daily,consecutively for 28 days.The open-field test was adopted to detect the total distance of autonomic movement of rats,and the pole climbing test was used to detect the body coordination ability of rats.In the substania nigra of midbrain,the positive expression of TH was determined using immunohistochemistry,the mRNA expression levels of α-syn,Sirt3,NLRP3 and GSDMD were detected by quantitative real-time fluorescence PCR,and the protein expression levels of NLRP3,apoptosis-associated speck-like protein containing a caspase-recruitment domain(ASC)and cysteinyl aspartate specific proteinase(Caspase)-1 were detected by Western blot.Results Compared with the control group,the total distance of autonomous movement was decreased(P<0.01)in the model group,and the score of pole climbing experiment was increased(P<0.01);in the midbrain substantia nigra the positive expression of TH was decreased(P<0.01);the mRNA expression level of Sirt3 was decreased(P<0.01),and those of α-syn,NLRP3 and GSDMD were increased(P<0.01);while the protein expression levels of NLRP3,ASC and Caspase-1 were increased(P<0.01).When compared with the model group,the total distance of autonomous movement in open field experiment was increased(P<0.01)in the EA group and the score of pole climbing experiment was lower(P<0.05);in the midbrain substantia nigra the positive expression of TH was increased(P<0.01);the mRNA expression level of Sirt3 in the midbrain substantia nigra was increased(P<0.01),and those of α-syn,NLRP3 and GSDMD were reduced(P<0.01);while the protein expression levels of NLRP3,ASC and Caspase-1 decreased(P<0.01,P<0.05).Conclusion EA at"GV16""LR3"and"ST36"can repair the neuronal injury,clear the abnormal accumulation of α-syn in the substania nigra of midbrain,and ameliorate mitochondrial damage in PD rats,which may be obtained by regulating Sirt3/NLRP3/GSDMD signaling pathway,so as to delay the occurrence and development of Parkinson's disease.关键词
帕金森病/电针/沉默信息调节因子同源蛋白3/NOD样受体3/消皮素DKey words
Parkinson's disease/Electroacupuncture/Sirtuin 3/NOD-like receptor 3/Gasdermin-D引用本文复制引用
汪瑶,王彦春,马骏..电针对帕金森病大鼠中脑黑质Sirt3/NLRP3/GSDMD信号通路的影响[J].针刺研究,2024,49(4):384-390,7.基金项目
国家自然科学基金项目(No.81473788) （No.81473788）
