电针对脑缺血再灌注学习记忆障碍大鼠海马脑源性神经营养因子转化和突触可塑性的影响OA北大核心CSTPCDMEDLINE

Objective To observe the effect of electroacupuncture(EA)at"Baihui"(GV20)and"Shenting"(GV24)on the rats'behavior and the transforming precursor of brain-derived neurotrophic factor(proBDNF)into mature brain-derived neurotrophic factor(mBDNF)in the hippocampus of rats with learning and memory impairment induced by cerebral ischemia-reperfusion(IR),so as to explore its mechanisms underlying improvement of learning and memory ability.Methods SD rats were randomly divided into blank,sham operation,model,and EA groups,with 6 rats in each group.The model of IR was established by occlusion of the middle cerebral artery.EA(1 Hz/20 Hz)was applied to GV24 and GV20 for 30 min,once daily for 14 days.The neurological function was evaluated according to the Zea Longa's score criteria 24 h after modeling and after intervention.Morris water maze test was used to detect the learning and memory function of the rats.TTC staining was used to evaluate the cerebral infarction volume on the affected side.The protein expression levels of proBDNF,mBDNF,tissue plasminogen activator(tPA),tyrosine kinase receptor B(TrkB)and p75 neurotrophin receptor(p75NTR)in hippocampal tissue were detected by Western blot.Results Compared with the sham operation group,the neurological function score,the percentage of cerebral infarction volume and the expression levels of proBDNF and p75NTR protein in hippocampus were increased(P<0.01),while the times of crossing the original platform and the total distance in the target quadrant,the expression levels of mBDNF,TrkB and tPA protein and the ratio of mBDNF/proBDNF were decreased(P<0.01,P<0.05)in the model group.Compared with the model group,the neurological function score,the percentage of cerebral infarction volume,and the expression levels of proBDNF and p75NTR protein in hippocampus were decreased(P<0.01,P<0.05),while the times of crossing the original platform,the total distance in the target quadrant,and the expression levels of mBDNF,TrkB and tPA protein and the ratio of mBDNF/proBDNF were increased(P<0.05,P<0.01)in the EA group.Conclusion EA can alleviate learning and memory impairment in IR rats,which may be related to its function in up-regulating the expression of tPA protein and promoting the transformation of proBDNF to mBDNF,thus improving the synaptic plasticity.