中国临床药理学杂志2024，Vol.40Issue(7)：1039-1043,5.DOI:10.13699/j.cnki.1001-6821.2024.07.022
黄芩素调控NLRP3炎性小体对痤疮的保护作用
Protective effects of baicalin regulating NLRP3 inflammasome against acne
摘要
Abstract
Objective To explore the protective mechanism of baicalin regulating NOD like receptor thermal protein domain associated protein 3(NLRP3)inflammasomes against acne.Methods Compound acne models were prepared by intradermal injection of Propionibacterium acnes into the auricle.Rats were randomly divided into control group(normal rats were given physiological saline by gavage),model group(acne model rats were given physiological saline by gavage),experimental-L,-M,-H groups(acne model rats were given 25,50,and 100 mg·kg-1 of baicalin by gavage),and positive control group(acne model rats were given 3.125 mg·kg-1 of isotretinoin by gavage),with 10 rats in each group.Observe the morphology of rat auricles;enzyme linked immunosorbent assay(ELISA)was used to detect the level of inflammation in serum;hematoxylin-eosin staining was used to detect pathological changes in rat auricle tissue;Western blotting was used to detect the protein expression level in the auricle tissue.Results After drug treatment,the auricular thickness of rats in the control,model,experimental-H and positive control groups were(0.42±0.05),(0.75±0.10),(0.49±0.05)and(0.50±0.05)mm;the serum levels of tumor necrosis factor-α were(20.46±2.13),(62.32±5.47),(23.27±2.26)and(25.41±2.28)pg·mL-1;interleukin-1 β levels were(11.38±1.26),(31.62±2.58),(15.61±1.35)and(16.72±1.38)pg·mL-1;interleukin-6 levels were(10.62±1.02),(25.43±2.51),(13.27±1.15)and(14.01±1.17)pg·mL-1;NLRP3 protein expression levels in auricular tissues were 0.23±0.03,0.81±0.08,0.30±0.04 and 0.32±0.04;and Caspase-1 protein expression levels were 0.31±0.04,0.76±0.08,0.39±0.04 and 0.41±0.04;matrix metalloproteinase-2 protein expression levels were 0.35±0.04,0.86±0.10,0.40±0.05 and 0.42±0.05.Compared with the model group,the above indexes in the experimental-H group were statistically significant(all P＜0.05).Conclusion Baicalin can inhibit the inflammatory response in acne rats,and its mechanism of action may be related to the inhibition of the NLRP3 inflammasome signaling pathway.关键词
黄芩素/痤疮/NOD样受体热蛋白结构域相关蛋白3炎性小体/炎症反应/基质金属蛋白酶-2蛋白Key words
baicalein/acne/NOD like receptor thermal protein domain associated protein 3 inflammatory vesicle/inflammatory response/matrix metalloproteinase-2 protein分类
医药卫生引用本文复制引用
毛俊涛,徐丽梅,曹牧,薛慧..黄芩素调控NLRP3炎性小体对痤疮的保护作用[J].中国临床药理学杂志,2024,40(7):1039-1043,5.基金项目
河北省中医药管理局科研计划基金资助项目(2023055) （2023055）
