用HPLC-MS/MS法测定重症感染患儿微量血浆/血清中利奈唑胺和万古霉素浓度OA北大核心CSTPCD
Determination of linezolid and vancomycin in trace plasma/serum of children with severe infection by HPLC-MS/MS
目的 建立一种高效液相色谱串联质谱(HPLC-MS/MS)法快速测定重症感染患儿微量血浆/血清中利奈唑胺和万古霉素浓度.方法 血浆/血清10 µL经甲醇沉淀蛋白后取上清液直接进样分析,内标分别为利奈唑胺-D3和去甲万古霉素;用 Kinetex® EVO C18 柱(30.0 mm × 2.1 mm,2.6 µm)分离,以0.1％甲酸-水和0.1％甲酸-乙腈为流动相,梯度洗脱,流速:0.5 mL·min-1,柱温:40℃,进样量:2μL,分析时长:…查看全部>>
Objective To establish a rapid high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the determination of linezolid and vancomycin in trace plasma/serum from pediatric patients with severe infection.Methods The plasma/serum specimens(10 μL)were precipitated by methanol,then the supernatant was injected for detection directly.The internal standards were linezolid-D3 and norvancomycin.The chromatographic separation was performed…查看全部>>
姜锡娟;雷蓉;秦亚彬;赵宜乐
河北省儿童医院 药学部,河北 石家庄 050031河北省药品医疗器械检验研究院河北省中药质量评价与标准研究重点实验室,河北石家庄 050227河北省儿童医院 药学部,河北 石家庄 050031河北省儿童医院 药学部,河北 石家庄 050031
药学
利奈唑胺万古霉素高效液相色谱-质谱联用治疗药物监测重症感染儿科患者
linezolidvancomycinhigh performance liquid chromatography-tandem mass spectrometrytherapeutic drug monitoringsevere infectionpediatric patients
《中国临床药理学杂志》 2024 (7)
1044-1048,5
河北省医学科学研究课题计划基金资助项目(20200658)
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