现代畜牧科技Issue(4)：1-6,6.DOI:10.19369/j.cnki.2095-9737.2024.04.001
马疱疹病毒1型UL56蛋白的原核表达及多克隆抗体的制备
Prokaryotic Expression and Polyclonal Antibody Preparation of UL56 Protein of Equid Alphaherpesvirus 1
摘要
Abstract
The purpose of this study is to prepare polyclonal antibody of pUL56 of Equid alphaherpesvirus 1.Based on the whole genome sequence of EHV-1 YM2019 strain in GenBank.The pUL56 was purified by Ni-NTA affinity chromatography,and BALB/c mice were immunized with Freund's adjuvant and pUL56 polyclonal antibody was prepared.The polyclonal antibody of the protein was measured by enzyme linked immunosorbent assay and Western blot.The results showed that the purified pUL56 could react with EHV-1 positive serum.The anti-pUL56 polyclonal antibody had a potency of 1∶128 000 and bound specifically to pUL56.In this study,the mouse anti-pUL56 polyclonal antibody had good reactogenicity,which laid the theoretical foundation for functional properties of pUL56 and the pathogenic mechanisms of virus.关键词
马疱疹病毒1型/UL56蛋白/原核表达/多克隆抗体Key words
Equid alphaherpesvirus 1/UL56 protein/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
张玉鉴,刘建华,撒瑞雪,吴桂灵,杨凯舒,张嗣玉,李银涛,齐晋卫,杨贤斌,邓智超..马疱疹病毒1型UL56蛋白的原核表达及多克隆抗体的制备[J].现代畜牧科技,2024,(4):1-6,6.基金项目
中央引导地方科技发展专项资金项目(ZYYD2023C03) （ZYYD2023C03）
新疆农业大学大学生创新训练计划项目(S202210758063) （S202210758063）
