中国医科大学学报2024，Vol.53Issue(3)：218-223,6.DOI:10.12007/j.issn.0258-4646.2024.03.005
长链非编码RNA-NRON对小鼠心肌梗死后细胞凋亡的作用机制研究
Effects of long noncoding RNA-NRON on apoptosis following myocardial infarction in mice
摘要
Abstract
Objective To investigate the effects of long noncoding RNA(lncRNA)-NRON on apoptosis following myocardial infarc-tion(MI)in mice.Methods The C57BL/6 mice were randomly divided into four groups:sham operation(Sham)group,MI group,MI combined with lncRNA-NRON interference lentivirus(MI+shNRON)group,and MI combined with the negative control(NC)lentivirus(MI+NC)group.The expression of lncRNA-NRON was detected using real-time PCR.In addition,the pathology of the myocardial tissue injury was analyzed using HE staining,the myocardial infarction size was examined using TTC staining,and the extent of apoptosis was assessed using the TUNEL assay,respectively.The RPISeq database was used to predict the probability of interaction between lncR-NA-NRON and the voltage-dependent anionic channel protein(VDAC).The effect of lncRNA-NRON on the expression of VDAC protein was detected using Western blotting.Results The lncRNA-NRON expression was significantly increased in the MI group,and the tar-geted knockdown of lncRNA-NRON resulted in alleviation of the pathological myocardial tissue injury,reduction in the myocardial infarc-tion area,and inhibition of apoptosis.The probability of interaction between lncRNA-NRON and VDAC reached 0.9,indicating a high probability of their association.Additionally,lncRNA-NRON could regulate the protein expression of VDAC.Conclusion Knockdown of lncRNA-NRON could reduce the occurrence of myocardial injury following myocardial infarction.This effect may be attributable to a spe-cific mechanism wherein lncRNA-NRON affects the process of apoptosis by binding to VDAC,consequently suppressing its expression.关键词
长链非编码RNA-NRON/心肌梗死/电压依赖性阴离子通道蛋白/细胞凋亡Key words
long noncoding RNA-NRON/myocardial infarction/voltage dependent anion channel/apoptosis分类
医药卫生引用本文复制引用
高涵,张春晶,李淑艳,师岩,郭红艳,杨超..长链非编码RNA-NRON对小鼠心肌梗死后细胞凋亡的作用机制研究[J].中国医科大学学报,2024,53(3):218-223,6.基金项目
黑龙江省省属高等学校基本科研业务费项目(2023-KYYWF-0860) （2023-KYYWF-0860）
黑龙江省卫生健康委医药卫生科研课题(20210202020361) （20210202020361）
齐齐哈尔医学科学院项目(QMSI2020B-04) （QMSI2020B-04）
齐齐哈尔医学科学院重点培育项目(2022-ZDPY-011) （2022-ZDPY-011）
齐齐哈尔市科技计划联合引导项目(LSFGG-2022057) （LSFGG-2022057）
